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| Screening and Fermentation Optimization of Corynebacterium acetoacidophilum Mutant for High-level Production of L-proline |
| LU Wei-ning1, LIAO Yu-ling1,2 |
1. Star Lake Bioscience Corporation Incorporated, Zhaoqing 526040,China;
2. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006,China |
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Abstract Corynebacterium acetoacidophilum was used as the original strain for stepwise mutation by whole genome fragmentation mutation (UV and NaNO2) and subsequent sulfaguanidine resistance screening. A L-proline over-producing mutant was obtained. The optimum contents of glucose, biotin and thiamin in shake-flask fermentation were 16%, 300μg/L and 400μg/L respectively, and pH is 6.8~7.0, where 25ml medium was cultured in 500ml shake-flask. After 72h by flask-shaking batch fermentation, the production of L-proline reached 75.6g/L, 5% higher than with the control. To investigate the effect of cell growth on the L-proline production, 50L fed-batch culture was performed at the specific growth rate (μ) of 0.06/h,0.08/h and 0.1/h.The results showed that the specific L-proline production rate reached 0.091g/(g.h) and the highest production was 82.1g/L, increased by 14% compared with the control when the specific growth rate (μ) was 0.08/h.
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Received: 15 July 2010
Published: 19 November 2010
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