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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (11): 1-5    DOI:
    
Recombinant Human Midkine Promotes the Repair of Partial Thickness Defects of Articular Cartilage in Rats
SUN Jiao-meng1, XU Chuan-ying2, ZHANG Zhong-hui1, WANG Jing1, YU Yan2, HAN Wei1
1. Laboratory of Regeneromics, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China;
2. Shanghai Municipality Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240,China
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Abstract  

Objective:To study the role of recombinant human midkine (rhMK) in the repair of acute partial-thickness defects of knee articular cartilage. Methods:The animal model of acute partial-thickness defects in rats was constructed, then normal saline or rhMK (20μg/kg、60μg/kg、180μg/kg)was injected into knee cavity 24 hours after surgery.All rats were sacrificed on the 8th week after surgery, knee joints were taken to make paraffin sections, then sections were stained with Toluidine blue and observed with microscope to determine optimum dose of injection.Results:Different dose of rhMK could repair partial-thickness defects in different degree, the optimum dose of injection was 180μg/kg. For pharmacokinetic analysis, cartilage was harvested at 1 hour and 1, 3, 6, 9, 12 and 15 days after knee injection of 180μg/kg rhMK (n=4), the concentration of rhMK in cartilage tissue firstly increased and then decreased, the terminal T1/2 in cartilage tissue was 8.69 days.Conclusion: rhMK could repair partial-thickness defects of knee articular cartilage in rats obviously, the optimum dose was 180μg/kg and the optimum interval of time was 8 days.



Key wordsRecombinant human midkine (rhMK)      Knee cartilage of rats      Optimum dose      Pharmacokinetics     
Received: 30 July 2010      Published: 19 November 2010
ZTFLH:  Q789  
Cite this article:

SUN Jiao-meng, XU Chuan-ying, ZHANG Zhong-hui, WANG Jing, YU Yan, HAN Wei. Recombinant Human Midkine Promotes the Repair of Partial Thickness Defects of Articular Cartilage in Rats. China Biotechnology, 2010, 30(11): 1-5.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I11/1

[1] 林家声,赵承斌.BMP/ bFGF对关节软骨损伤修复的作用.中国伤残医学杂志,2009,17(2):131-133. Lin J S, Zhao C B. Chinese Journal of Trauma and Disability Medicine, 2009,17(2):131-133.
[2] Tsutsui J, Kadomatsu K, Matsubara S, et al. A new family of heparin-binding growth differentiation factors: increased midkine expression in Wilms' tumor and other human carcinomas. Cancer Res, 1993, 53 (6): 1281-1285.
[3] Garver R I Jr, Radford D M, Donis-Keller H, et al. Midkine and pleiotrophin expression in normal and malignant breast tissue. Cancer, 1994, 74 (5): 1584-1590.
[4] Namba R S, Meuli M, Sullivan K M, et al. Spontaneous repair of superficial defects in articular cartilage in a fetal lamb model. J Bone Joint Surg, 1998, 80(1): 4-10.
[5] Yamamoto T, Wakitani S, Imoto K, et al. Fibroblast growth factor-2 promotes the repair of partial thickness defects of articular cartilage in immature rabbits but not in mature rabbits. Osteoarthritis Cartilage, 2004, 12 (8): 636-641.
[6] Masaya H, Takeshi M, Young-Jin J, et al. Weekly intra-articular injections of bone morphogenetic protein-7 inhibits osteoarthritis progression. Arthritis Research & Therapy, 2008, 10(5):R118.
[7] Redman S N, Oldfield S F, Archer C W. Current strategies for articular cartilage repair. Eur Cell Mater, 2005,14(9):23-32.
[8] Saraf A, Mikos A G. Gene delivery strategies for cartilage tissue engineering. Adv Drug Deliv Rev, 2006, 58 (4):592-603.
[9] Brittberg M, Lindahl A, Nilsson A, et al. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Eng J Med, 1994, 331 (14): 889-895.
[10] Zhang Y, An H S, Tannoury C, et al. Biological treatment for degenerative disc disease. Am J Phys Med Rehabil, 2008, 87 (9): 694-702.
[11] Lattermann C, Oxner W M, Xiao X, et al. The adeno associated viral vector as a strategy for intradiscal gene transfer in immune competent and pre-exposed rabbits. Spine, 2005, 30 (5): 497-504.
[12] Sugiyama O, An D S, Kung S P, et al. Lentivirus-mediated gene transfer induces long-term transgene expression of BMP-2 in vitro and new bone formation in vivo. Mol Ther, 2005, 11 (3): 390-398.
[13] Bieback K, Kern S, Kluter H, et al. Critical parameters of the isolation of mesenchymal stem cells from umbilical cord blood. Stem Cells, 2004, 22 (4): 625-634.
[14] Lee O K, Kuo T K, Chen W M, et al. Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood, 2004, 103 (5): 1669-1675.
[15] Zhang Y, Li Z, Thonar E J, et al. Transduced bovine articular chondrocytes affect the metabolism of cocultured nucleus pulposus cells in vitro: implications for chondrocyte transplantation into the intervertebral disc. Spine, 2005, 30 (23): 2601-2607.
[16] Darling E M, Athanasiou K A. Rappid phenotypic changes in passaged articular chondrocyte subpopulations. J Orthop Res, 2005, 23 (2): 425-432.
[17] Tuli R, Tuli S, Nandi S, et al. Transforming growth factor-betamediated chondrogenesis of human mesenchymal progenitor cells involves N-cadherin and mitogen-activated protein kinase and Wnt signaling cross-talk. J Biol Chem, 2003, 278 (42): 41227-41236.
[18] Fortier L A, Mohammed H O, Lust G, et al. Insulin-like growth factor-I enhances cell-based repair of articular cartilage. J Bone Joint Surg Br, 2002, 84 (2): 276-288.

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