|
|
|
| Development of Chimera Protein: sFcεRIα/mIg(IgG2) |
| MA Jin-wei, CHENG Hong, CHU Xue-zhe, LI Xian-zhong, HU Yan-hong, YU Zhi-ai, LIN Feng, HE Li-hua, BAI Xian-hong, HU Pin-liang |
| Biotech Pharmaceutical Ltd. Beijing 100176,China |
|
|
|
Abstract IgE plays a key role in type I hypersensitivity reactions. The blocks of IgE binding to its high affinity receptor FcεRI can efficiently eliminate allergic reaction. The sFcεRIα/mIg(IgG2)was constructed by gene fusion of the extracellular portion of α subunit of FcεRI receptor with human IgG2 hing region, CH2 and CH3 region. The chimera could be a markedly longer plasma half-life than the soluble extracellular domain. This chimeric protein was produced by transfecting Chinese Hamster Ovary cells (CHO). Following affinity purification, the apparent molecular weight of sFcεRIα/mIg(IgG2), which showed high affinity with human and mouse IgE, was above 170 kDa. A good foundation for industrialization of this new engineered soluble protein for type I hypersensitivity diseases was provided.
|
|
Received: 29 September 2009
Published: 25 October 2010
|
|
|
|
Corresponding Authors:
HU Pin-liang
E-mail: 327hu@sina.com
|
|
Cite this article:
MA Jin-wei, CHENG Hong, CHU Xue-zhe, LI Xian-zhong, HU Yan-hong, YU Zhi-ai, LIN Feng, HE Li-hua, BAI Xian-hong, HU Pin-liang. Development of Chimera Protein: sFcεRIα/mIg(IgG2). China Biotechnology, 2010, 30(10): 17-21.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I10/17
|
|
|
|
[1] Tokunaga K H,Yoshida C,Suzuki K M,et al. Antihypertensive effect of peptides from royal jelly in spontaneously hypertensive rats. Biol Pharm Bull,2004,27(2):189-192.
[2] Kolbinger F,Saldanha J,Hardman N,et al. Humanization of a mouse anti-human IgE antibody: a potential therapeutic for IgE-mediated allergies. Protein Eng,1993,6(8):971-980.
[3] Rigby L J,Trist H,Snider J,et al. Monoclonal antibodies and synthetic peptides define the active site of FcepsilonRI and a potential receptor antagonist. Allergy,2000,55(7):609-619.
[4] Rudolf M P,Zuercher A W,Nechansky A,et al. Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody. J Immunol,2000,165(2):813-819.
[5] Naito K,Hirama M,Okumura K. Recombinant soluble form of the human high-affinity receptor for IgE prevents anaphylactic shock in mice. J Allergy Clin Immunol,1996,97(3):773-780.
[6] Gavin A L,Snider J,Hulett M D,et al. Expression of recombinant soluble Fc epsilon RI: function and tissue distribution studies. Immunology,1995,86(3):392-398.
[7] 萨姆布鲁克 J,弗里奇 E F,曼尼阿蒂斯 T. 分子克隆实验指南. 第2版,北京:科学出版社,1992. 888-897. Sambrook J, Fritsch E F,Maniatis T. Molecular Cloning A Laboratory Manual. 2nd ed,Beijing:Science Press,1992. 888-897.
[8] Garman S C,Wurzburg B A,Tarchevskaya S S,et al. Structure of the Fc fragment of human IgE bound to its high-affinity receptor Fc epsilonRI alpha. Nature,2000,406(6793):259-266.
[9] Hsu D H,Shi J D,Homola M,et al. A humanized anti-CD3 antibody, HuM291, with low mitogenic activity, mediates complete and reversible T-cell depletion in chimpanzees. Transplantation,1999,68(4):545-554.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
