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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (05): 36-42    DOI:
    
Construction and Immuno-protection Evaluation of Trivalent-epitope DNA Vaccine pVIVO2-SjFABP/Sj26.SjGAPDH
ZHU Lu1,2,LONG Quan-ke1,2,LIU Hai-feng1,2,LU Ming-bo1,2 ,HU Yuan3,SHI You-en3,YU Long-jiang1,2
1.Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan 430074, China
2.College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
3.Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
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Abstract  

Objective: To construct trivalent-epitope DNA vaccine encoding SjFABP, SjGAPDH and Sj26, and evaluate the protective immunity efficacy against Schistosoma japanicum by pharmacological trials. Methods: The monovalent antigen gene SjFABP, SjGAPDH and Sj26 were obtained by RT-PCR. Sj26 and SjGAPDH are spliced into covalent fusion gene of Sj26.SjGAPDH by recombinant PCR. pVIVO2-SjFABP/Sj26.SjGAPDH is constructed by subcloning the SjFABP and Sj26.SjGAPDH into mcs1 and mcs2 of pVIVO2-mcs respectively. The expression of fusion antigen protein in MCF-7 cell line transfected with pVIVO2-SjFABP/Sj26.GAPDH was determined by indirect immunofluorescence assay (IIF) and reverse transcriptase-polymerase chain reaction (RT-PCR). The evaluation of vaccine’s immuno-protection efficacy is tested by pharmacological trials. Results: The trivalent-epitope DNA vaccine pVIVO2-SjFABP/Sj26.SjGAPDH was successfully constructed. In the trivalent-epitope DNA vaccine group, the worm reduction rate and the egg reduction rate were 58.6% and 59.8% compared with control group. Conclusion: The trivalent-epitope DNA vaccine pVIVO2-SjFABP/Sj26.SjGAPDH could induce higher protection efficiency than both monovalent and bivalent vaccine, and paves the way for development of DNA vaccine against Schistosoma japanicum.



Key wordsSchistosama japanicum      SjFABP      SjGAPDH      Sj26      Trivalent-epitope DNA vaccine     
Received: 30 December 2009      Published: 25 May 2010
Corresponding Authors: Longjiang Yu     E-mail: yulj@mail.hust.edu.cn
Cite this article:

SHU Lu, LONG Quan-Ke, LIU Hai-Feng, LU Meng-Bei, HU Yuan, DAN You-En, TU Long-Jiang. Construction and Immuno-protection Evaluation of Trivalent-epitope DNA Vaccine pVIVO2-SjFABP/Sj26.SjGAPDH. China Biotechnology, 2010, 30(05): 36-42.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I05/36

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