Over-expression and Detection Analysis of Myostatin in Chicken Embryos Mediated by Retrovirus

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China Biotechnology ›› 2010, Vol. 30 ›› Issue (05) : 23-26.

MAN Chao-lai,LI Xiang

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Abstract

Chick embryos have become a great experimental model system for developmental biology, immunology and cancer,etc. The chicken embryo and myostatin are used as model system and target gene respectively. The process of transferring gene into chicken embryo and detection are researched using retrovirus-mediated gene transfer and in situ hybridization. The experimental results show that target gene myostatin can be integrated into the embryonic genome, and has transcriptional activity;Whole-mount in situ hybridization can accurately reflect the virus integration sites and target gene expression levels.The study not only provides a base for further study myostatin functions in embryonic development, but also is a methodology attempt.

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Chick / Embryo / Retrovirus / In Situ Hybridization

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MAN Chao-Lai, LI Xiang. Over-expression and Detection Analysis of Myostatin in Chicken Embryos Mediated by Retrovirus[J]. China Biotechnology, 2010, 30(05): 23-26

References

［1］ Stern C D. The chick:a great model system becomes even greater.Dev Cell,2005,8(1):917.
［2］ Brown W R, Hubbard S J, Tickle C, et al.The chicken as a model for largescale analysis of vertebrate gene function. Nat Rev Genet,2003,4(2):8798.
［3］ Sazanov A, Ewald D, Buitkam P J, et al. A molecular marker for the chicken Myostatin gene (GDF8) maps to 7p11. Anim Genet, 1999, 30(5):388389.
［4］ Mcpherron A C, Lawler A M, Lee S J. Regulation of skeletal muscle mass in mice by a new TGFbeta superfamily member. Nature, 1997, 387(6628): 8390.
［5］ Szabo G, Dallmann G, Muller G, et al. A deletion in the Myostatin gene causes the compact (Cmpt) hypermuscular mutation in mice. Mamm Genome,1998, 9(8):671672.
［6］ Armand A S, Della Gaspera B, Launay T, et al. Expression and neural control of follistatin versus myostatin genes during regeneration of mouse soleus. Dev Dyn,2003, 227(2):256265.
［7］ Reardon K A, Davis J, Kapsa R M, et al. Myostatin, insulinlike growth factor1, and leukemia inhibitory factor mRNAs are upregulated in chronic human disuse muscle atrophy. Muscle Nerve, 2001, 24(7):893899.
［8］满朝来,陈岩.逆转录病毒法制备转基因鸡的方法分析.中国农学通报,2007,23(8):17. Man C L,Chen Y.Chinese Agricultural Science Bulletin, 2007,23(8):17.
［9］ Streit A, Stern C D. Combined wholemount in situ hybridization and immunohistochemistry in avian embryos.Methods,2001,23(4):339344.
［10］ Soneoka Y,Cannon P M, Ramsdale E E,et al.A transient three plasmid expression system for the production of high titer retroviral vectors.Nucleic Acids Research, 1995,23(4):628633.
［11］ Morissette M R, Cook S A, Foo S, et al.Myostatin regulates cardiomyocyte growth through modulation of Akt signaling. Circulation Research,2006,99(1):1524.
［12］ Saxena V K, Sundaresan N R, Malik F,et al. Temporal expression of transforming growth factorβ2 and myostatin mRNA during embryonic myogenesis in Indian broilers. Research in Veterinary Science,2007, 82(1):5053.
［13］ Kubota K, Sato F,Aramaki S,et al. Ubiquitous expression of myostatin in chicken embryonic tissues:Its high expression in testis and ovary. Comparative Biochemistry and Physiology, Part A,Motecular and Integrative Physiology,2007,148(3):550555
［14］ Sundaresan N R,Saxena V K,Singh R,et al.Expression profile of myostatin mRNA during the embryonic organogenesis of domestic chicken(Gallus gallus domesticus).Res Vet Sci,2008,85(1):8691.
［15］ Lee S G, Kim S, Robbins P D, et al. Optimization of environmental factors for the production and handing of recombinant retrovirus.Appl Microbiol Biotechnol,1996,45(4):477483.