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| Over-expression and Detection Analysis of Myostatin in Chicken Embryos Mediated by Retrovirus |
| MAN Chao-lai,LI Xiang |
| College of Life Science and Technology, Harbin Normal University, Harbin 150025,China |
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Abstract Chick embryos have become a great experimental model system for developmental biology, immunology and cancer,etc. The chicken embryo and myostatin are used as model system and target gene respectively. The process of transferring gene into chicken embryo and detection are researched using retrovirus-mediated gene transfer and in situ hybridization. The experimental results show that target gene myostatin can be integrated into the embryonic genome, and has transcriptional activity;Whole-mount in situ hybridization can accurately reflect the virus integration sites and target gene expression levels.The study not only provides a base for further study myostatin functions in embryonic development, but also is a methodology attempt.
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Received: 09 December 2009
Published: 25 May 2010
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Corresponding Authors:
Man Chaolai
E-mail: manchaolai@126.com
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