Study on Adsorption Properties of Lycopene from the Broth of Blakeslea trispora by Macroporous Resin

China Biotechnology

2010, Vol. 30

Issue (04): 71-76 DOI:

Study on Adsorption Properties of Lycopene from the Broth of Blakeslea trispora by Macroporous Resin

LI Gan-lu,WU Qi-ci,WU Zhen-jun,SHI Nan,WAN Hong-gui

Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009,China

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Abstract

The potential of macroporous resin for separation and purification of lycopene produced by B.trispora were investigated. The adsorptiondesorption properties for lycopene of the resins such as HP20, HP2MG, SP825 and SP207 were studied. The optimal resin for lycopene was screened and selected. The results expressed that HP20 resin had a better adsorption-desorption property for lycopene. It could quickly reach an adsorption balance in one hour. Purification of lycopene using packed bed column by HP20 resin was carried out using step gradient of 55% isopropyl alcohol in acetone followed by 65% isopropyl alcohol in acetone. A 89.55% recovery of lycopene was detected by HPLC. After further recrystallisation the purity of lycopene was above 95% and the structure was confirmed by IR, MS and 1H-NMR. The whole process was simple, easy to operate, with a good industrial application value.

Key words： Blakeslea trispora Lycopene Macroporous resin Step gradient elution

Received: 16 December 2009 Published: 29 April 2010

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	LI An-Lu
	TUN Qi-Ci
	WU Zhen-Jun
	DAN Nan
	MO Gong-Gui

Cite this article:

LI An-Lu, TUN Qi-Ci, WU Zhen-Jun, DAN Nan, MO Gong-Gui. Study on Adsorption Properties of Lycopene from the Broth of Blakeslea trispora by Macroporous Resin. China Biotechnology, 2010, 30(04): 71-76.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I04/71

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