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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (04): 120-124    DOI:
    
Transcription Mediated Amplification and Its Applications in Diagnosis
GAO Shan-dian,CHANG Hui-yun,DU Jun-zheng,CONG Guo-zheng,SHAO Jun-jun,LIN Tong
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, National FMD Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China
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Abstract  

Transcription Mediated Amplification is a target nucleic acid amplification method that uses RNA polymerase and reverse transcriptase to produce RNA amplicon from a target RNA or DNA under isothermal condition at about 42℃. TMA produces 100~1000 copies per cycle which results in a 10 billion fold increase of copies within about 15~30 minutes. TMA is now widely used in genotyping of human leukocyte antigen and diagnosis of many bacterium and viruses.



Key wordsTranscription mediated amplification      Isothermal amplification      Diagnosis     
Received: 25 June 2009      Published: 29 April 2010
Cite this article:

GAO Shan-Dian, CHANG Hui-Yun, DU Jun-Zheng, CONG Guo-Zheng, SHAO Jun-Jun, LIN Tong. Transcription Mediated Amplification and Its Applications in Diagnosis. China Biotechnology, 2010, 30(04): 120-124.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I04/120

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