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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (04): 116-119    DOI:
    
Overview on Promoter of Glucoamylase Gene glaB from Aspergillus oryzae
LIU Lu,XING Pei-pei,ZUO Zu-zhen,SHI Bi-hong
College of Life Science, Fujian Normal University, Fuzhou 350108, China
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Abstract  

Aspergillus oryzae expression system has many advantages when compared to E.coli and yeast expression system. At present the vast majority of  studies focus on how to improve the production of protein by A. oryzae in liquid submerged fermentation.However, in solid culture conditions, the ability of A. oryzae to produce a variety of proteins was stronger than in the liquid culture conditions. This is not only related to growth patterns of A. oryzae under different culture conditions, but also with the characteristics of regulation factors, such as promoters, which control the specific gene in different metabolic pathways. The expression efficiency of A. oryzae glucoamylase gene, glaB, under solid culture conditions was significantly higher than that of the liquid culture conditions. The glucoamylase gene was used as an example and the studies of glaB promoter were overviewed, which provide a reference for making better use of such promoters in the future.



Key wordsAspergillus oryzae      Solid-state fermentation      Glucoamylase-encoding genesgla      B promoter     
Received: 30 December 2009      Published: 29 April 2010
Cite this article:

LIU Lu, GENG Pei-Pei, ZUO Jie-Zhen, SHI Bi-Gong. Overview on Promoter of Glucoamylase Gene glaB from Aspergillus oryzae. China Biotechnology, 2010, 30(04): 116-119.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I04/116

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