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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (03): 40-45    DOI:
    
Screening and Identification of Differentially Expressed Genes in Beijing Fatty Chicken and Broiler Adipose Tissue
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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Abstract  

Differential display reverse transcription PCR(DDRT-PCR) was used to identify differential expression genes in Beijing fatty chicken and Arbor Acres Broiler(AA) adipose tissue. A total of 10 ESTs were found using DDRT-PCR and reverse northern dot blot, all of 10 ESTs were compared with nucleotide sequences deposited in the nr database and Gallus database. XF2、YF1、YF2 and YF4 had their highly similar nucleotide sequences in nucleotide databases but with unknown functions. XF4 is similar to full-length cDNA clone CL0BA010ZF08 of Placenta of Homo sapiens, the identity is 83%; YF3 was found highly similar to MLL5 (LOC417712),but the function is unknow; XF5 and YF5 were highly similar to HMGN3; XF3 had no significant similarity with existing genes or ESTs and was regarded as new EST. The new EST was submitted to GenBank(Accession number: EU594549). The lays a foundation for further study on the mechanism of differential gene expression in Beijing fatty and AA adipose tissue.



Key wordsBeijing chicken      Arbor acres broiler      DDRT-PCR      Adipose tissue     
Received: 12 October 2009      Published: 25 March 2010
Corresponding Authors: Miao XiangYang     E-mail: mxy32@sohu.com
Cite this article:

MIAO Xiang-Yang, FENG Gao-Yong. Screening and Identification of Differentially Expressed Genes in Beijing Fatty Chicken and Broiler Adipose Tissue. China Biotechnology, 2010, 30(03): 40-45.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I03/40

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