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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (03): 105-111    DOI:
    
Advances on Cyanobacteria Genetic Engineering Applications
1.Department of Biopharmaceuticals, School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102,China
2.School of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin 300450,China
3.Institute of Botany, Chinese Academy of Sciences, Beijing 100093,China
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Abstract  

Cyanobacteria are used as a host of gene engineering to express foreign gene, their unique properties gradually attracting scientists' attention. The stable cyanobacteria transformation system has been constructed since 1990. In the past 20 years, more than 30 foreign genes have been successfully expressed in cyanobacteria. With the further investigation, cyanobacteria transgenic expression system has the potential to be extensively used for production of recombinant drugs, clean energy resources,  farm chemical and for preventing the environment pollution et al. This review summed up the development of cyanobacteria genetic engineering and their uses in medicine, environment, agriculture and biosensor. Then, this review analyzed the development bottlenecks of the cyanobacteria transgenic engineering, the low expression rate of the foreign gene in cyanobacteria. Finally，it summarized the efforts that many scientists have made to improve the low expression rate, including: choosing high efficient gene and oral preparation, optimize transcription and translation component, adjust the host cell`s physiological state, et al.

Key words： Cyanobacteria      Genetic Engineering      Expression system     
Received: 21 October 2009      Published: 25 March 2010
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XI Chao
WANG Chun-Mei
SHI Ding-Ji
Cite this article:

XI Chao, WANG Chun-Mei, SHI Ding-Ji. Advances on Cyanobacteria Genetic Engineering Applications. China Biotechnology, 2010, 30(03): 105-111.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I03/105

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