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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (02): 84-88    DOI:
    
Identification of an Endophytic Paenibacillus polymyxa Strain Producing Antifungal Protein and the Inhibition to Fusarium moniliforme causing Rice Bakanae Disease
Institute of Microbiology of Heilongiang Academy of Sciences, Harbin 150010,China
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Abstract  

An antagonistic endophytic bacterium, designated as strain SD-6,was isolated from healthy tomato leaves using dual culture method. Based on phenotypical and biochemical properties as well as its 16S rRNA gene sequence, the bacterium was identified as Paenibacillus polymyxa. In vitro antifungal spectrum assay vindicate that it was active against a broad range of phytopathogens and inhibited the growth of thirteen fungal pathogens. In order to further evaluate the biocontrol potential against rice bakanae disease caused by Fusarium moniliforme, crude protein was extracted from fermentation broth using ammonium sulphate precipitation. The crude protein inhibited the mycelial growth and spore germenation of F.moniliforme, and caused distortion, tumescence of a portion of the germinated spores. This is the first report about isolating an antagonistic endophytic P.polymyxa from tomato leaves, which produced antifungal protein and possessed the inhibitory activity against a wide range of fungal pathogens. The protective properties of selected P.polymyxa strain make it as potential tool to reduce deleterious impact of fungal diseases. The strain SD-6 and associated proteins are potentially useful in controlling rice bakanae disease.



Key wordsPaenibacillus polymyxa      Endophytic bacteriaIdentification      Antagonistic activity      Fusarium moniliforme     
Received: 18 November 2009      Published: 26 February 2010
Cite this article:

ZHANG Chu-Mei, SHA Chang-Jing, DIAO Xiao-Yu, WANG Yu-Xia, ZHANG Xian-Cheng, LI Jing, MENG Li-Jiang, XU De-Shui. Identification of an Endophytic Paenibacillus polymyxa Strain Producing Antifungal Protein and the Inhibition to Fusarium moniliforme causing Rice Bakanae Disease. China Biotechnology, 2010, 30(02): 84-88.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I02/84

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