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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (01): 93-97    DOI:
    
Application of Microbial Protoplast Fusion Technology in Genetic Breeding
MAO Yu1,WANG Dan2,3,HUANG Zhan-bin1,XING Jian-min2
1.School of Chemical and Evironmental Engineering, China University of Mining&Technology, Beijing 100083, China
2.National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
3.Graduate University of Chinese Academy of Sciences, Beijing 100049, China
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Abstract  

Strain breeding is very important in fermentation industry. Compared with other breeding methods, microbial protoplast fusion technology(mPFT) has advantages such as high recombination frequency, little limitation by genus or sex, and complete transfer of genetic material. Now mPFT has became one of the most staple methods in micro-organisms breeding. Here the procedures of mPFT in combination with the relational study in recent years was reviewed, including the factors that affected the formation, regeneration, fusion of protoplasts, and the ways of selecting fusants. Furthermore, the latest application of mPFT in micro-organisms breeding and the prospect of mPFT were discussed.



Key wordsmicroorganism      protoplast fusion      genetic breeding      genome shuffling     
Received: 09 September 2009      Published: 27 January 2010
Cite this article:

MAO Yu, WANG Dan, HUANG Tie-Bin, GENG Jian-Min. Application of Microbial Protoplast Fusion Technology in Genetic Breeding. China Biotechnology, 2010, 30(01): 93-97.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I01/93

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