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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (01): 67-72    DOI:
    
The Establishment of Scale-up Isolation Procedure of Phycoerythrin and Phycocyanin from Porphyra yezoensis
LI Chun-xia,WU Shu-xian,CAI Chun-er,WANG Qing,CHEN Si-hong,LI Hui,HE Pei-min
Aqual-life Science and Technology College,Shanghai Ocean University,Shanghai 201306,China
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Abstract  

In this paper, the way of “extracting－salting－chromatography” was used to purify the phycoerythrin and phycocyanin from Porphyra yezoensis in process scale-up. First, by comprehensive comparison of efficiency, the Sephadex G-25 was selected from four resins (Sephadex G-25、G-100、S-300 and CL-6B) as the best choice used in crude extract desalting of phycobiliprotein. Then the preparation process of phycobiliprotein was scaled-up with raw material(Porphyra yezoensis) increased from 1g to 20g,and finally to 400g. The results indicated that the yields of purified phycoerythrin and phycocyanin (absorption spectra purity above 3.2) increased during according to process scale-up, with 0.323% phycoerythrin and 0.148% phycocyanin obtained from 400g frozen Porphyra yezoensis blades respectively. It is no doubt that the process involved in the experiment is a potential way for large scale preparation of phycobiliproteins of high purity.

Key words： Porphyra yezoensis      Phycoerythrin      Phycocyanin      Sephadex G-25      Scale-up     
Received: 03 September 2009      Published: 27 January 2010
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LI Chun-Xia
TUN Chu-Xian
CA Chun-Er
HONG Qing
CHEN Sai-Hong
LI Hui
HE Pei-Min
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LI Chun-Xia, TUN Chu-Xian, CA Chun-Er, HONG Qing, CHEN Sai-Hong, LI Hui, HE Pei-Min. The Establishment of Scale-up Isolation Procedure of Phycoerythrin and Phycocyanin from Porphyra yezoensis. China Biotechnology, 2010, 30(01): 67-72.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I01/67

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