Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (01): 51-55    DOI:
    
Gene expression analysis of a late embryogenesis abundant gene from Salvia miltiorrhiza Bunge
LIU Cong-ling,WANG Zhe-zhi
Colledge of Life Scences, Shaanxi Normal University, Key Laboratory of Medicinal Plant Resources and Natural Pharmaceutical Chemistry,Ministry of Education,National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, Xi’an 710062,China
Download: HTML   PDF(739KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

According to the sequence of SmLEA gene, a DNA fragment of 1 038 bp upstream of the coding sequence of SmLEA gene was amplified by DNA walking with the genomic DNA of Salvia miltiorrhiz as the template. Sequence analysis showed that the fragment contained some putative ciselements relating to abiotic stress, ABA, seed specific expression.So the S.miltiorrhiz seedings was treated with 100μmol/L ABA,200mmol/L NaCl,4℃, and subjected to dehydration. The real-time PCR showed that expression levels of SmLEA was increased obviously, which was in accordance with the sequence analysis..



Key wordsS. miltiorrhiza      SmLEA gene      promoter      expression pattern     
Received: 22 September 2009      Published: 27 January 2010
Cite this article:

LIU Cong-Ling, WANG Zhe-Zhi. Gene expression analysis of a late embryogenesis abundant gene from Salvia miltiorrhiza Bunge. China Biotechnology, 2010, 30(01): 51-55.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I01/51

[1] Yancey P H, Clark M E, Hand S C, et al. Living with water stress: evolution of osmolyte systems. Science,1982,217(4566): 12141222. 
[2] Dure Ⅲ L, Greenway S C, Galau G A. Developmental biochemistry of cottonseed embryogenesis and germination: changing messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesis. Biochemistry,1981, 20(14): 41624168. 
[3] Kim H S, Lee J H, Kim J J, et al. Molecular and functional characterization of CaLEA6, the gene for a hydrophobic LEA protein from Capsicum annuum.Gene, 2005,344(10): 115123. 
[4] Maitra N, Cushman J C. Isolation and characterization of a droughtinduced soybean cDNA encoding a D95 family lateembryogenesisabundant protein. Plant Physiol,1994, 106(2): 805806. 
[5] Battista J R, Park M J, McLemore A E. Inactivation of two homologues of proteins presumed to be involved in the desiccation tolerance of plants sensitizes Deinococcus radiodurans R1 to desiccation. Cryobiology,2001,43(2): 133139. 
[6] Stacy R A P, Aalen R B. Identification of sequence homology between the internal hydrophilic repeated motifs of Group 1 lateembryogenesisabundant proteins in plants and hydrophilic repeats of the general stress protein GsiB of Bacillus subtilis. Planta,1998,206(3): 476478. 
[7] Sales K, Brandt W, Rumbak E, et al. The LEAlike protein HSP 12 in Saccharomyces cerevisiae has a plasma membrane location and protects membranes against desiccation and ethanolinduced stress. Biochim Biophys Acta, 2000,1463 (2): 267278. 
[8] Honjoh K I, Matsumoto H, Shimizu H, et al. Cryoprotective activities of group 3 late embryogenesis abundant proteins from Chlorella vulgaris C27. Biosci Biotechnol Biochem, 2000,64 (8): 16561663. 
[9] Browne J, Tunnacliffe A, Burnell A. Anhydrobiosis: plant desiccation gene found in a nematode. Nature, 2002, 416(6876): 38. 
[10] Gal T Z, Glazer I, Koltai H. Differential gene expression during desiccation stress in the insectkilling nematode Steinernema feltiae IS6. J Parasitol,2003, 89(4): 761766. 
[11] Gal T Z, Glazer I, Koltai H. An LEA group 3 family member is involved in survival of C.elegans during exposure to stress. FEBS Lett,2004, 577(12):2126. 
[12] Sivamani E, Bahieldin1 A, Wraith J M, et al. Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene.Plant Sci, 2000, 155(1): 19. 
[13] Imai R, Chang L, Ohta A, et al. A leaclass gene of tomato confers salt and freezing tolerance when expressed in Saccharomyces cerevisiae. Gene, 1996, 170 (2): 243248. 
[14] 路静, 赵华燕, 何奕昆. 高等植物启动子及其应用研究进展. 自然科学进展, 2004,14(8): 856862. Lu J,Zhao H Y,He Y K.Progress in Natural Science, 2004,14(8): 856862. 
[15] 刘霞, 邓馨. 复苏植物牛耳草BhLEA2基因启动子的分离和基因表达模式研究. 自然科学进展, 2009,19(1): 3944. Liu X,Deng X.Progress in Natural Science,2009,19(1): 3944. 
[16] 刘丛玲, 王喆之. 丹参晚期胚胎富集蛋白基因SmLEA的克隆及表达分析. 生物技术通报, 2009,5: 8084. Liu C L,Wang Z Z.Biotechnology Bulletin, 2009,5: 8084. 
[17] Stalberg K, Ellerstom M, Ezcurra I, et al. Disruption of an overlapping Ebox/ABRE motif abolished high transcription of the napA storageprotein promoter in transgenic Brassica napus seeds. Planta ,1996 ,199(4) :515519. 
[18] Song J,Wang Z. Molecular cloning, expression and characterization of a phenylalanine ammonialyase gene(SmPAL1)from Salvia miltiorrhiza. Mol Biol Rep,2009,36(5):939952. 
[19] 刘国宝, 郑易之. 大豆种子Em基因(LEA1) 启动子的克隆与序列分析. 大豆科学, 2007,26 (4): 454459. Liu G B,Zheng Y Z. Soybean Science, 2007,26 (4): 454459. 
[20] 罗克明, 郭余龙, 肖月华. 棉花Lea蛋白D 113基因启动子的克隆及序列分析. 遗传学报, 2002, 29 (2) :161165. Luo K M ,Guo Y L , Xiao Y H . Acta Genetica Sinica, 2002, 29 (2) :161165. 
[21] George S, Usha B, Parida A. Isolation and characterization of an atypical LEA protein coding cDNA and its promoter from droughttolerant plant Prosopis juliflora. Appl Biochem Biotechnol,2009, 157(2):244253. 
[22] Kaplan B, Davydov O, Knight H, et al. Rapid transcriptome changes Induced by cytosolic Ca2+ transients reveal ABRErelated sequences as Ca2+responsive cis elements in Arabidopsis. Plant Cell, 2006, 18(10): 27332748. 
[23] Hobo T, Asada M, Kowyama Y, et al. ACGTcontaining abscisic acid response element (ABRE) and coupling element 3 (CE3) are functionally equivalent. Plant J,1999 ,19(6):679689. 
[24] Zhang W, Ruan J, Ho T H, et al. Cisregulatory element based targeted gene finding: genomewide identification of abscisic acid and abiotic stressresponsive genes in Arabidopsis thaliana. Bioinformatics,2005, 21(14):30743081. 
[25] Abe H, Urao T, Ito T, et al. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell, 2003, 15(1): 6378. 
[26] Park H C, Kim M L, Kang Y H, et al. Pathogen and NaClinduced expression of the SCaM4 promoter is mediated in part by a GT1 box that interacts with a GT1like transcription factor. Plant Physiology, 2004, 135(4):21502161. 
[27] Kim S Y, Chung H J, Thomas T L. Isolation of novel class of bZIP transcription factors that interact with ABArespinsive and embryospecification elements in the Dc3 promoter using a modified yeast one hybrid system.The Plant Journal,1997,11(6):12371251. 
[28] Kawagoe Y, Murai N. Four distinct nuclear proteins recognize in vitro the proximal promoter of the bean seed storage protein βphaseolin gene conferring spatial and temporal control. The Plant Journal,1992, 2(6):927936. 
[29] Allen R D, Bernier F, Lessard P A, et al. Nuclear factors interact with a soybean betaconglycinin enhancer. Plant Cell,1989, 1(6):623631.

[1] BU Kai-xuan,ZHOU Cui-xia,LU Fu-ping,ZHU Chuan-he. Research on the Regulation Mechanism of Bacterial Transcription Initiation[J]. China Biotechnology, 2021, 41(11): 89-99.
[2] ZHU Ya-xin, DUAN Yan-ting, GAO Yu-hao, WANG Ji-yue, ZHANG Xiao-mei, ZHANG Xiao-juan, XU Guo-qiang, SHI Jin-song, XU Zheng-hong. Synthesis and Regulation of Poly-γ-glutamic Acid with Different D/L Monomer Ratios[J]. China Biotechnology, 2021, 41(1): 1-11.
[3] XUAN Mei-juan,ZHANG Xiao-yun,GAO Ying,Li-GAO Ying,WU Jia-jing,MA Mei,WANG Yan-mei,KOU Hang,LU Fu-ping,LI Ming. Characterization of Promoters in the Glycolytic Pathway and Tricarboxylic Acid Cycle of E. coli and Its Application[J]. China Biotechnology, 2020, 40(6): 20-30.
[4] HU Yi-bo,PI Chang-yu,ZHANG Zhe,XIANG Bo-yu,XIA Li-qiu. Recent Advances in Protein Expression System of Filamentous Fungi[J]. China Biotechnology, 2020, 40(5): 94-104.
[5] SHI Chao-shuo,LI Deng-ke,CAO Xue,YUAN Hang,ZHANG Yu-wen,YU Jiang-yue,LU Fu-ping LI Yu. The Effect on Heterologous Expression of Alkaline Protease AprE by Two Different Promoter and Combinatorial[J]. China Biotechnology, 2019, 39(10): 17-23.
[6] HUANG Yu,HUANG Shu-ting,ZHANG Xi-mei,LIU Yan. Cloning and Functional Analysis of the Promoter of HSP70 Gene in Gobiocypris rarus[J]. China Biotechnology, 2019, 39(10): 9-16.
[7] Ya-fang LI,Ying-hui ZHAO,Sai-bao LIU,Wei WANG,Wei-jun ZENG,Jin-quan WANG,Hong-yan CHEN,Qing-wen MENG. Chicken OV Promoter Expressed HA to Protect Chickens from Lethal Challenge of AIV[J]. China Biotechnology, 2018, 38(7): 67-74.
[8] Jia-zhen WANG,Lun-guang YAO,Feng WANG,Yun-chao KAN,Jin-ping LUO,Qian-qian HUANG,Jian-ping DUAN. Cloning and Activity Analysis of a Midgut-specific Promoter in Silkworm (Bombyx mori)[J]. China Biotechnology, 2018, 38(2): 13-17.
[9] ZHANG Ling,WANG Nan,JIN Lv-hua,LIN Rong,YANG Hai-lin. To Promote the Expression of Leucine Dehydrogenase in Bacillus subtilis via Dual-Promoter and Fermentation Research[J]. China Biotechnology, 2018, 38(12): 21-31.
[10] Peng HUANG,Li-ping YAN,Ning ZHANG,Jin-lei SHI. Constitutive Expression of Human Goose-type Lysozyme 2 in Pichia pastoris Using the GAP Promoter[J]. China Biotechnology, 2018, 38(10): 55-63.
[11] Wen-juan CHAI,Qi YANG,Guo-jing LI,Rui-gang WANG. CiMYB15 from Caragana Intermedia Positively Regulates Flavonoids Metabolism of Arabidopsis[J]. China Biotechnology, 2018, 38(10): 8-19.
[12] NIE Yong-qiang, MA Hai-yan, MA Qing-wen. An in vivo Robust System for Generation of Site-specific Integration Minicircle DNA Vector[J]. China Biotechnology, 2017, 37(7): 80-87.
[13] XIA Hui, LIU Lei, WANG Xiu, SHEN Yan-qiu, GUO Yu-lun, LIANG Dong. Research on Stress-inducible Expression Characteristics of Sorbitol-6- phosphate Dehydrogenase Promoter from Apple[J]. China Biotechnology, 2017, 37(6): 50-55.
[14] NI Xuan, GAO Jin-xin, YU Chuan-jin, LIU Tong, LI Ya-qian, CHEN Jie. Bioinformatic Analysis and Promoter Identification of clt-1 Gene in Curvularia Lunata[J]. China Biotechnology, 2017, 37(3): 37-42.
[15] LUO Feng-xue, LI Fo-sheng, YAO Min, XU Ying. The Cloning and Transient Expression Analysis of Promoter of OsHAK26 from Oryza sativa[J]. China Biotechnology, 2017, 37(2): 33-39.