中国生物工程杂志

［1］ Kagi D, Ledermann B, Burkl K, et al. Cytotoxicity mediated by T cells and natural killer cells is greatly impaired in perforindificient mice. Nature, 1994, 369:3137. 
［2］ Nakajima H, Fujiwara I, Matsuda T, et al. Perforin/granzymes, pathway operates in xenogeneic human antipig cytotoxicity. Transplant Proc, 1998, 30:7678. 
［3］ 秦卫松. 穿孔素氨基端肽段的原核表达、抗体制备和信使核糖核酸的定量检测.上海：第二军医大学,2001. Qin W S. Expression, Purification, Antibodies Preparation of Human Perforin Nterminal Peptide and Quantification of Perforin mRNA.Shanghai:Second Military Medical University,Clinical Testing and Diagnostics, 2001. 
［4］ Sutton R B, Davletov B A, Berghuis A M, et al. Structure of the first C2 domain of synaptotagmin I: a novel Ca2+/ phospholipidbinding fold. Cell, 1995, 80:929938. 
［5］ Pipkin M E, Lieberman J. Delivering the kiss of death: progress on understanding how perforin works. Curr Opin Immunol, 2007, 19:301308. 
［6］ Gebhard J R, Perry C M, Harkins S, et al. Coxsackievirus B3 induced myocarditis: perforin exacerbates disease, but plays no detectable role in virus clearance. Am J Pathol, 1998, 153:417 428. 
［7］ Smyth M J, Thia K Y, Street S E. Perforinmediated cytotoxicity is critical for surveillance of spontaneous lymphoma. J Exp Med, 2000, 192(5):755760. 
［8］ Liu C C, Persechini P M, Young J D. Expression and characterization of functionally active recombinant perforin produced in insect cells. J Immunol, 1996, 156 :32923300. 
［9］ Mullbacher A, Hla R T, Museteanu C, et al. Perforin is essenltial for control of ectromelia virus but not related poxviruses in mice. J Virol, 1999, 73(2):16651667. 
［10］ Matloubian M, Suresh M, Glass A, et al. A role for perforin in downregulating Tcell responses during chronic viral infection. J Virol, 1999, 73(3):25272536. 
［11］ Nalefski E A, Falke J J. The C2 domain calciumbinding motif: structural and functional diversity. Protein Sci, 1996, 5(12):23752390. 
［12］ Pappa H, MurrayRust J, Dekker L V, et al. Crystal structure of the C2 domain from protein kinase Cdelta. Structure, 1998, 6(7):885894. 
［13］Uellner R, Zvelebil M J, Hopkins J, et al. Perforin is activated by a proteolytic cleavage during biosynthesis which reveals a phospholipidbinding C2 domain. EMBO J, 1997, 16(24):72877296. 
［14］ Voskoboinik I, Thia M C, Fletcher J, et al. Calciumdependent plasma membrane binding and cell lysis by perforin are mediated through its C2 domain: A critical role for aspartate residues 429, 435, 483 and 485 but not 491. J Biol Chem, 2005, 280(9):84268434. 
［15］ Uellner R, Zvelebil M J, Hopkins J, et al. Perforin is activated by a proteolytic cleavage during biosynthesis which reveals a phospholipidbinding C2 domain. EMBO J, 1997,16: 72877296. 
［16］ 周向阳, 李芳秋, 武建国. 人穿孔素羧基端肽段的表达纯化与活性鉴定. 南京大学学报(自然科学版), 2001, 37(2): 246251. Zhou X Y, Li F Q, Wu J G.Journal of NanJing University (Natural Sciences), 2001, 37(2): 246251. 
［17］ 王立锋. 穿孔素基因的克隆、表达及其凋亡诱导活性研究.西安：第四军医大学, 2008. Wang L F. Cloning, expression and apoptosis inducing activity of perforin.Xi’an:Fourth Military Medical University, 2008.