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| Study on Rapidly Simultaneous Detection of Deoxynivalenol and Zearalenone |
| ZHANG Jue1,GAO Lei2,ZHOU Bin1,ZHANG Yi1,HUANG Biao1,JIN Jian2 |
1.Key Laboratory of Nuclear Medicine,Ministry of Health,Jiangsu Key Laboratory of Molecular Nuclear Medicine,Jiangsu Institute of Nuclear Medicine，Wuxi 214063, China
2.School of Medicine and Pharmaceutics, Southern Yangtze University, Wuxi 214063, China |
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Abstract Objective: A high sensitive indirect competitive dual-label time-resolved fluoroimmunoassay (TRFIA) was established to simultaneously detect deoxynivalenol and Zearalenone in cereal. Methods: DON-BSA and ZEN-BSA were co-coated in 96-well plates as the solid phase antigen to compete with free DON and ZEN for limited anti-DON polyclonal antibody or anti-ZEN monoclonal antibody. The antibodies of goat anti-rabbit and goat anti-mouse were labeled with the Eu3+- and Sm3+-chelates respectively, used as the tracers to establish DON/ZEN dual-label TRFIA in dissociation enhanced fluorescence immunoassay system. Results: For this method, the sensitivity was 0.2 ng/mL for DON and 0.7 ng/mL for ZEN. The assay ranges were 0.2 -100 ng/mL for DON and 0.7-50 ng/mL for ZEN, with intra- and inter-assay CV less than 10%. The average recovery for DON was 102.8% in corn and 98.8% in wheat samples, while average recovery for ZEN was 94.2% in corn and 95.7% in wheat samples. In DON/ZEN dual-label TRFIA, there was no interaction between DON and ZEN. The results obtained by the presented assay were in good agreement with those by single- label assay with the correlation ratio of 0.9760 for DON and of 0.9695 for ZEN. Conclusion: The DON/ZEN dual-label TRFIA can be served as a high sensitivity, wide measuring range, good stability method for large scale samples and has a wide application prospect.
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Received: 06 August 2009
Published: 07 December 2009
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