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| Bridge PCR,An Easy Way for Concatemerizing DNA Tags |
| MAO Jian-ping**,WANG Quan-hui,ZHOU Ying,FANG Jing,CUI Yu-fang |
| Institute of Radiation Medicine,Beijing 100850, China |
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Abstract In MAST (mRNA accessible site tagging), the DNA tags from synthesized library were employed for identifying mRNA accessible sites. A large number of tags were amplified and subcloned for sequencing to verify mRNA binding profiles. A PCR was designed by using one primer which bridges over the tag terminal sequences. In PCR reaction DNA tag fragments were concatemerized by a bridge primer in reaction cycles. The concatemerized tag fragments were subcloned and sequenced. Dozens of the concatemerized sequences contained thousands tags. The PCR was a simple, effective way which for sequencing tags in a high through put manner
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Received: 03 August 2009
Published: 07 December 2009
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