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| Application of Thermal Shift Assays in Purification of Recombinant TBEV Serine Protease |
| ZHU Jun-ping,FAN Dong-ying |
| School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China |
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Abstract Thermal Shift Assays was applied for high-throughput screening optimized buffer system used in the gel filtration chromatography for purification of recombinant TBEV serine protease. The optimization included kinds of salt and pH. It was optimized new buffer remarkably improved the quality of the purified TBEV serine protease by increasing its monomer from 75% to 99%. This result may improve future crystal studies of this protein.
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Received: 08 July 2009
Published: 07 December 2009
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Corresponding Authors:
Jun-Ping ZHU
E-mail: jpzhu0217@vip.sina.com
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