中国生物工程杂志

Objective: To study the feasibility of using the bionic technology to construct small-caliber vascular prostheses with modified SIS . Methods: The vascular endothelial cells and smooth muscle cells were separated from canine saphenous artery, the cells blended with collagen gel, which were planted respectively on the SIS films, these films were rolled into the biologic three-layer prostheses around a 3mm diameter polyethylene tube; one-layer prostheses without these cells and collagen gel served as control. These 2 types of prostheses were implanted into the defect of bilateral canine femoral by anastomosis in 15 dogs. doppler colour ultrasonic, histology detection and electron microscope examination were done postoperatively. Results: By 12 weeks postoperatively, 14 biologic vascular prostheses had kept well patency, the patency rate being 93.3%, the biologic structure like blood vessels had formed, the inner surface of the vessels had been covered with full endothelial cells, a lot of smooth muscle cells had been found in the media of vascular walls in regular line; the patency rate in control group was 60.0%, the endothelial cell coverage was incomplete. Conclusions: The bionic vascular prostheses showed potent blood compatibility, which could keep long-term patency in vivo.Curative effect of repairing the small-caliber vessel defect was well satisfactory.