Optimization of Fermentation Conditions for High Expression Productivity of Recombinant TRAIL

China Biotechnology

2009, Vol. 29

Issue (10): 74-80 DOI:

Optimization of Fermentation Conditions for High Expression Productivity of Recombinant TRAIL

BAO Xi-Jun,WEI Dong-zhi¹,SHEN Ya-ling¹,ZHOU Jin-song²,ZHANG Guo-jun²,SUN Jing²,QIU Yi²

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Abstract

Tumor necrosis factorrelated apoptosisinducing ligand(TRAIL) is a novel anticancer therapeutic agent. In order to reduce the incubation time and enhance the production efficiency, firstly the response surface method was used to optimize the fermentation and expression condition of TRAIL in shake flasks. As a result, the expression of the total TRAIL protein reached 25.7%. Based on this, the influence of fermentation condition to the soluble TRAIL protein expression was studied. The recombinant strains were cultured in 3.7L fermenter and the expression of soluble TRAIL protein per gram cell enhanced 67%. That realized the high soluble expression of TRAIL protein per gram cell in high cell density and the enhancement of volume productivity.

Key words： Apo2L/TRAIL Recombinant Escherichia coli Response surface Fermentation optimization

Received: 23 April 2009 Published: 29 October 2009

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	BAO Xi-Jun
	WEI Dong-Zhi
	CHEN E-Ling
	ZHOU Jin-Song
	ZHANG Guo-Jun
	SUN Jing
	QIU Yi

Cite this article:

BAO Xi-Jun, WEI Dong-Zhi, CHEN E-Ling, ZHOU Jin-Song, ZHANG Guo-Jun, SUN Jing, QIU Yi. Optimization of Fermentation Conditions for High Expression Productivity of Recombinant TRAIL. China Biotechnology, 2009, 29(10): 74-80.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I10/74

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