The Constitutive Expression  of CALB in Pcihia pastoris and Purification of Product

China Biotechnology

2009, Vol. 29

Issue (10): 55-59 DOI:

The Constitutive Expression of CALB in Pcihia pastoris and Purification of Product

ZHU Yan-fei¹,YANG Bo¹

School of Bioscience & Bioengineering,South China University of Technology,Guanzhou 510006,China

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Abstract

The artificially synthetic gene calb(only mature peptide sequence) was inserted into a yeast constitutive vector, pGAPZαA. Under the control of the promoter GAP(glyceraldehydes 3phosphate dehydrogenase)CALB was expressed constitutively. The yeast transformants still had the foreign gene after ten times of passage in YPD medium.The activity of CALB in shake flask cultured 4 days achieved 14.5U/ml.And after two steps of purification (the desalinization and DEAE column), purity of the final protein achieved 95% and protein concentration has achieved 220mg/L.

Key words： lipase B from Candida antarctica(CALB) Pichia pastoris Constitutive expression Purification

Received: 14 May 2009 Published: 29 October 2009

ZTFLH:

Q786

Corresponding Authors: YANG Bo E-mail: yanbo@scut.edu.cn

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Cite this article:

SHU Yan-Fei, YANG Bo. The Constitutive Expression of CALB in Pcihia pastoris and Purification of Product. China Biotechnology, 2009, 29(10): 55-59.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I10/55

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