P300 Enhances Nanog Transcriptional Activity Through the Homeobox Domain

China Biotechnology

2009, Vol. 29

Issue (10): 1-5 DOI:

P300 Enhances Nanog Transcriptional Activity Through the Homeobox Domain

1.State Key Laboratory of Biomembrane and Membrane Biotechnology,Institutes of Biomedicine,School of Medicine,Tsinghua University,Beijing 100084,China
2.Key Laboratory of Regenerative Biology,Guangzhou Institute of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510663,China
3.National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beiming Forestry University,Beijing 100083,China.

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Abstract

Embryonic stem cells (ES cells) undergo selfrenewal to maintain their pluripotency. The selfrenewal and pluripotency of ES cells are under tight control of many transcription factors. Nanog is one of these transcription factors. However, the molecular mechanisms under which Nanog regulates ES cell pluripotency are not very clear. P300 is a general coactivator in eukaryotes which usually helps transcription factors to regulate downstream genes. Nanog (or Nanog mutants) and its reporter gene were cotransfeted with p300 in cells in order to see if p300 could affect Nanog transcriptional activity. The results showed that p300 enhances Nanog transcriptional activity through the homeobox domain.

Key words： ES cell Nanog p300 homeobox domain transcriptional activity

Received: 05 May 2009 Published: 29 October 2009

ZTFLH:

Q756

Corresponding Authors: Yun-Qian GUO E-mail: pei_duanqing@gibh.ac.cn

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	GUO Yuan-Qian
	CHEN Mo
	MA Tian-Hua
	FEI Duan-Qing

Cite this article:

GUO Yuan-Qian, CHEN Mo, MA Tian-Hua, FEI Duan-Qing. P300 Enhances Nanog Transcriptional Activity Through the Homeobox Domain. China Biotechnology, 2009, 29(10): 1-5.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I10/1


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