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| Study on Electrotransformation of vitro DNA into Pseudomonas putida |
| DENG Ting-Ting1, LI Hui1, LIU Yan2, ZHENG Yuan-Yuan2, LI Chun1,2 |
1.Agricultural College of Shihezi University/Key Laboratory for Green Processing of Xinjiang Bingtuan,Shihezi832003,China
2.School of Life Science and Technology, Beijing Institute of Technology, Beijing100081,China |
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Abstract Pseudomonas putida isolated relieving salt stress and promoting plant growth was used as the recipient strain. Optimum conditions including growth stage of the strain, concentration of competent cell, electroshock voltage and the meduim were investigated for the electroporation of P.putidaE.coli shulter vector PDSK519 into P.putida Rs198. It was showed that the highest transformation efficiency was up to 1.3×107CFU/μ g DNA in the optimium condition, under which the competent cells were collected at logarithmic growth phase(OD600=0.5), the mixture of the competent cells(4.6×1012/ml) and PDSK519 plasmid DNA was electroporated at 13kV/cm. It will be very helpful for developing a genetic transformation system and studying the genomic function of P.putida.
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Received: 10 March 2009
Published: 28 July 2009
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Corresponding Authors:
Deng TingTing
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