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| Cloning and bioinformatics analysis of Fgf9, a novel gene related to sex determination in cow |
| LIAO Bing1, TUN Ning1,2, HAN Feng-Dong3, LIN Xiu-Kun1 |
1.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing100094|China
2.Institute of Oceanology, Chinese Academy of Science,Qingdao266071,China
3.Northeast Agricultural University, Harbin150030, China |
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Abstract Fgf9 is the most important signal factor implicated in mediating the proliferation of Sertoli cells and the cord formation in testis morphogenesis of vertebrate. With the cloning principle of expressed sequence tags, sequence splicing and RTPCR were applied in the cloning of novel gene from Holstein cow and analyzing its expression pattern in various cow tissues. The bioinformatics analysis on the sequence and protein results of Fgf9 was conducted. Results indicated that Fgf9 was located at cow 12 chromosome, with fullsequence of 697bp. The open reading frame is 627bp in length and encodes a deduced amino acid sequence of 208 residues. The molecular weight of Fgf9 protein is 23.38245kDa, and its pI is 7.0600. RTPCR demonstrated the correctness of its ORF. Fgf9 was expressed in each tissue. No obvious homology with other cow cDNA was found for Fgf9.The GenBank accession number EU693028 was achieved. Function and structural analysis indicated that Fgf9 has a typical FGFs conserved domains, receptor interaction site and heparin binding site. It was predicted that Fgf9 has no signal peptide.
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Received: 14 January 2009
Published: 28 July 2009
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Corresponding Authors:
Liao Bing
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