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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (08): 113-118    DOI:
    
The New Technique for Protein Analyses:Proximity Ligation  Assay and Its Application
 TANG Na1, SHEN Zhi-Jiang1,2, GUAN Yu1, WANG Jin-Liang1
1. Key Laboratory of Preventive Veterinary Medicine and Animal Biotechnology, Binzhou Animal Husbandry and Veterinary Research Institute in Shandong, Binzhou256600, China
2. Lüdu Biological Technology Co., Ltd, Binzhou256600,China
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Abstract  

Proximity ligation assay (PLA) is a recently developed strategy for protein analysis in which a pair of proximity probes are designed to bind pairwise to target proteins via a DNA ligation reaction of oligonucleotides results in the formation of an amplifiable DNA strand suitable for analysis. It allows extremely sensitive and specific detection of  proteins by allowing the protein targets to be represented as DNA signatures that may be subsequently amplified and detected by methods like PCR. Here the basic principle and the potential applications of PLA were discussed, as well as the preparation of proximity probes.



Key wordsproximity ligation assay;proximity probe;protein analysis;diagnosis     
Received: 10 February 2009      Published: 28 July 2009
ZTFLH:  Q51  
Cite this article:

TANG Na, CHEN Zhi-Jiang, GUAN Yu, WANG Jin-Liang. The New Technique for Protein Analyses:Proximity Ligation  Assay and Its Application. China Biotechnology, 2009, 29(08): 113-118.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I08/113

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