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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (08): 107-112    DOI:
    
The Construction Strategy for Tandem Polypeptide Genes
 ZHANG Jun, YANG Ya-Lin, TIAN Zi-Gang, WANG Jian-Hua
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Abstract  

The tandem expression vector can effectively increase the expression level and structure stability of the target peptides (protein) with small size of molecular, and avoid toxicity towards host cell from some toxic peptides product, therefore, this approach is widely applied in biotechnology. The four construction methods of tandem expression plasmid including asymmetric and complementary cohesive ends, directional adapter, isocaudarners, and tandem expression cassette were reviewed in terms of protocol, characteristic and applicable field. In addition, selection principles from various construction methods of tandem plasmid were reviewed in terms of efficiency, accuracy and product cleavage.



Key wordsPeptide;Multimers;Multi-copy;Tandem;Construction     
Received: 19 March 2009      Published: 28 July 2009
ZTFLH:  Q819  
Corresponding Authors: Jianhua WANG   
Cite this article:

ZHANG Jun, YANG Ya-Lin, TIAN Zi-Gang, WANG Jian-Hua. The Construction Strategy for Tandem Polypeptide Genes. China Biotechnology, 2009, 29(08): 107-112.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I08/107

[1] Gottlieb C T, Thomsen L E, Ingmer H, et al. Antimicrobial peptides effectively kill a broad spectrum of Listeria monocytogenes and Staphylococcus aureus strains independently of origin, subtype, or virulence factor expression. BMC Microbiol, 2008, 8(1): 205
[2] Lee S J, Lee J H, Jin H J, et al. A novel technique for the effective production of short peptide analogs from concatameric short peptide multimers. Mol Cells, 2000, 10(2): 236~240
[3] Kim J M, Jang S A, Yu B J, et al. Highlevel expression of an antimicrobial peptide histonin as a natural form by multimerization and furinmediated cleavage. Appl Microbiol Biotechnol, 2008, 78(1): 123~130
[4] Xiao L Q, Liu A H, Zhang Y L. An effective method for raising antisera against betadefensins: doublecopy protein expression of mBin1b in E. coli. Acta Biochim Biophys Sin (Shanghai), 2004, 36(8): 571~576
[5] Wei Q, Kim Y S, Seo J H, et al. Facilitation of expression and purification of an antimicrobial peptide by fusion with baculoviral polyhedrin in Escherichia coli. Appl Environ Microbiol, 2005, 71(9): 5038~5043
[6] Lennick M, Haynes J R, Shen S H. Highlevel expression of alphahuman atrial natriuretic peptide from multiple joined genes in Escherichia coli. Gene, 1987, 61(1): 103~112
[7] Reed S I. Preparation of productspecific antisera by gene fusion: antibodies specific for the product of the yeast celldivisioncycle gene CDC28. Gene, 1982, 20(2): 255~265
[8] Lee J H, Skowron  PM, Rutkowska S M, et al. Sequential amplification of cloned DNA as tandem multimers using classIIS restriction enzymes. Genet Anal, 1996, 13(6): 139~145
[9] Shen S H. Multiple joined genes prevent product degradation in Escherichia coli. Proc Natl Acad Sci U S A, 1984, 81(15): 4627~4631
[10] Rao X C, Hu J, Li S, et al. Design and expression of peptide antibiotic hPABbeta as tandem multimers in Escherichia coli. Peptides, 2005, 26(5): 721~729
[11] Jeong D W, Shin D S, Ahn C W, et al. Expression of antihypertensive peptide, HisHisLeu, as tandem repeats in Escherichia coli. J Microbiol Biotechnol, 2007, 17(6): 952~959
[12] Park C J, Lee J H, Hong S S, et al. Highlevel expression of the angiotensinconvertingenzymeinhibiting peptide, YG1, as tandem multimers in Escherichia coli. Appl Microbiol Biotechnol, 1998, 50(1): 71~76
[13] Tian Z G, Teng D, Yang Y L, et al. Multimerization and fusion expression of bovine lactoferricin derivative LfcinB15W4,10 in Escherichia coli. Appl Microbiol Biotechnol, 2007, 75(1): 117~124
[14] Wang Y Q ,Cai J Y. Highlevel expression of acidic partnermediated antimicrobial peptide from tandem genes in Escherichia coli. Appl Biochem Biotechnol, 2007, 141(23): 203~213
[15] Mauro J M, Pazirandeh M. Construction and expression of functional multidomain polypeptides in Escherichia coli: expression of the Neurospora crassa metallothionein gene. Lett Appl Microbiol, 2000, 30(2): 161~166
[16] Tian Z G, Dong T T, Yang Y L, et al. Expression of antimicrobial peptide LH multimers in Escherichia coli C43(DE3). Appl Microbiol Biotechnol, 2009, doi: 10.1007/s002530091893z
[17] Hartley J L, Gregori T J. Cloning multiple copies of a DNA segment. Gene, 1981, 13(4): 347~353
[18] Li C, Ng M L, Zhu Y, et al. Tandem repeats of Sushi3 peptide with enhanced LPSbinding and neutralizing activities. Protein Eng, 2003, 16(8): 629~635
[19] Jiang S W, Trujillo M A, Eberhardt N L. An efficient method for generation and subcloning of tandemly repeated DNA sequences with defined length, orientation and spacing. Nucleic Acids Res, 1996, 24(16): 3278~3279
[20] Pan A, Tie F, Yang M, et al. Construction of multiple copy of alphadomain gene fragment of human liver metallothionein IA in tandem arrays and its expression in transgenic tobacco plants. Protein Eng, 1993, 6(7): 755~762
[21] Taylor W H, Hagerman  PJ. A general method for cloning DNA fragments in multiple copies. Gene, 1987, 53(2~3): 139~144
[22] 汪小福,刘仁虎,陈笑芸,等. 可剪切多拷贝抗菌肽融合表达载体的构建. 遗传, 2007, 29(6): 758~764
Wang X F, Liu R H, Chen X Y, et al. Hereditas, 2007, 29(6): 758~764
[23] Kajino T, Takahashi H, Hirai M, et al. Efficient production of artificially designed gelatins with a Bacillus brevis system. Appl Environ Microbiol, 2000, 66(1): 304~309
[24] Zhong Z, Xu Z, Peng L, et al. Tandem repeat mhBD2 gene enhance the soluble fusion expression of hBD2 in Escherichia coli. Appl Microbiol Biotechnol, 2006, 71(5): 661~667
[25] 喻光荣,孙学铮,沈卫英,等. hCGβCTP37多聚体在巴斯德毕赤酵母中的表达及其表达产物的结构预测. 实验生物学报, 2005, 38(2): 157~163
Yu G R, Sun X Z, Shen W Y, et al. Acta Biologiae Experimentalis Sinica, 2005, 38(2): 157~163
[26] Lewis R V, Hinman M, Kothakota S, et al. Expression and purification of a spider silk protein: a new strategy for producing repetitive proteins. Protein Expr Purif, 1996, 7(4): 400~406
[27] Arrigo S J, Huffman K. Potent inhibition of human immunodeficiency virus type 1 (HIV1) replication by inducible expression of HIV1 PR multimers. J Virol, 1995, 69(10): 5988~5994
[28]Lee J H, Kim M S, Cho J H, et al. Enhanced expression of tandem multimers of the antimicrobial peptide buforin II in Escherichia coli by the DEADbox protein and trxB mutant. Appl Microbiol Biotechnol, 2002, 58(6): 790~796
[29] Mansur M, Cabello C, Hernandez L, et al. Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris. Biotechnol Lett, 2005, 27(5): 339~345
[30] 梁伟锋,张朝春,杨希才. 一种多拷贝毕赤酵母表达载体的构建及人脑源性神经营养因子的表达. 微生物学报, 2005, 45(1): 34~38
Liang W F, Zhang Z C, Yang X C. Acta Microbiologica Sinica, 2005, 45(1): 34~38
[31] 井申荣,邹全明,洪俞, 等. 白细胞介素10基因多拷贝表达盒的构建及在毕赤酵母中的表达. 中国生物制品学杂志, 2007, 20(2): 81~86
Jing S R, Zou Q M, Hong Y, et al. Chinese Journal of Biologicals, 2007, 20(2): 81~86
[32] Kim S C, Szybalski W. Amplification of cloned DNA as tandem multimers using BspMIgenerated asymmetric cohesive ends. Gene, 1988, 71(1): 1~8
[33] Li Y, Li X, Wang G. Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL37 in Escherichia coli for NMR studies. Protein Expr Purif, 2006, 47(2): 498~505
[34] Li L, Wang J X, Zhao X F, et al. High level expression, purification, and characterization of the shrimp antimicrobial peptide, Chpenaeidin, in Pichia pastoris. Protein Expr Purif, 2005, 39(2): 144~151

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