|
|
|
| Optimal Model Design for High Production of Phenazine-1-Caboxylic Acid (PCA) |
|
|
|
Abstract Model on secondary metabolite phenazine-1-carboxylic acid (PCA) fermentation nutrition conditions of gacA inactivate mutant Pseudomonas sp. M18G was constructed by Plackett-Burman design (PB) and Response Surface Method (RSM). In PB design four key components selected from 12 different factors have shown to play an important role for promoting PCA production. Center Composite Design (CCD) was adopted to establish a fermentation model for the four nutrition components using RSM. The optimal concentration of the four components based on analysis of regression equation were determined that soybean meal 33.4g/L , glucose 12.7g/L, soy peptone10.9g/L, ethanol13.8g/L, and the highest PCA production could reached 1.89g/L after 60h fermentation and the yield increased to 6 fold over that before optimization. The contour graphs depicted interactions of the two nutrition components showed that soybean meal and ethanol played an even more crucial role for the highest production of PCA in fermentation.
|
|
Received: 04 March 2009
Published: 02 July 2009
|
|
|
| ［1］ Mavrodi D V, Blankenfeldt W ,Thomashow L S. Phenazine compounds in fluorescent pseudomonas spp. biosynthesis and regulation. Annu Rev Phytopathol, 2006, 44 (5): 417~445
［2］ Laursen J B, Nielsen J. Phenazine natural products: biosynthesis, synthetic analogues, and biological activity. Chem Rev, 2004, 104 (3):1663~1685
［3］ Lau G W, Hassett D J, Ran H, et al. The role of pyocyanin in Pseudomonas aeruginosa infection. Trends Mol Med, 2004, 10 (12): 599~606
［4］ Mavrodi D V, Bonsall R F, Delaney S M, et al. Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1. J Bacteriol, 2001, 183 (21): 6454~6465
［5］ Hu H B, Yu Q X, Feng C, et al. Isolation and characterization of a new fluorescent Pseudomonas strain that produces both phenazine 1-carboxylic acid and pyoluteorin. J Microbiol Biotechn, 2005, 15 (1): 86~90
［6］ 许煜泉. 绿色微生物源杀菌剂申嗪霉素（M18）. 精细和专用品化学, 2004, 20(12): 8~10
Xu Y Q.Fine and Specialty Chemicals, 2004, 20 (12): 8~10
［7］ Ge Y, Huang X, Wang S, et al. Phenazine-1-carboxylic acid is negatively regulated and pyoluteorin positively regulated by gacA in Pseudomonas sp. M18. FEMS Microbiol Lett, 2004, 237 (7): 41~47
［8］ Khambhaty Y, Mody K, Jha B, et al. Statistical optimization of medium components for kappa-carrageenase production by Pseudomonas elongata. Enzyme Microb Tech, 2007, 40(4): 813~822
［9］ Kennedy M, Krouse D. Strategies for improving fermentation medium performance: a review. J Ind Microbiol Biot, 1999, 23 (6): 456~475
［10］ Gheshlaghi R, Scharer J M, Moo-Young M, et al. Medium optimization for hen egg white lysozyme production by recombinant Aspergillus niger using statistical methods. Biotechnol Bioeng , 2 005, 90 (6): 754~760
［11］ Duffy B K, Defago G. Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Appl Environl Microb, 1999, 65 (6): 2429~2438
［12］ Raaijmakers J M, Vlami M, de Souza J T. Antibiotic production by bacterial biocontrol agents. Antonie van Leeuwenhoek, 2002, 81(11): 537~547
［13］ Shtark O, Shaposhnikov A I, Kravchenko L V. The production of antifungal metabolites by Pseudomonas chlororaphis grown on different nutrient sources. Mikrobiologiia , 2003, 72 (5): 645~650
［14］ Xiao Z J, Liu P H, Qin J Y, et al. Statistical optimization of medium components for enhanced acetoin production from molasses and soybean meal hydrolysate. Appl Microbiol Biotechnol , 2007, 74(1): 61~68
［15］ Slininger P J, Shea-Wilbur M A. Liquid-culture pH, temperature, and carbon (not nitrogen) source regulate phenazine productivity of the take-all biocontrol agent Pseudomonas fluorescens 2-79. Appl Microbiol Biotechnol, 1995, 43 (5): 794~800
［16］ van Rij E T, Wesselink M, Chin-A-Woeng T F C, et al. Influence of environmental conditions on the production of phenazine-1-carboxamide by Pseudomonas chlororaphis PCL1391. Mol Plant Microbe In, 2004, 17(5): 557~566
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
