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| Study on the lectin of endophytic fungus isolated from Pinellia ternata（Thunb．）Breit |
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Abstract Pinellia ternate (Thunb.) Breit is widely used as a kind of traditional Chinese herb for more than two thousands years in China. In our research, endophytic fungus were isolated from Pinellia ternate (Thunb.) Breit. Total proteins were extracted by organic solvent from the fermentation products of the endophytic fungus. The agglutination activity of proteins extracted from the endophytic fungus were studied by erythrocyte of rabbit, and the result showed that the protein from strains gs1 exhibited high lectin activity. The lectin extracted from the strains gs1 was purified by Mannan-Sepharose 4B affinity column. It was indicated by SDS-PAGE that the molecular of the lectin was about 12 kDa. The saccharide binding specificities were studied. The lectin from the strains gs1 showed high affinity with mannose. It was measured by Brandford method that there was 9.58 mg lectin per 1000 ml cultures from the the strains gs1. The agglutination activity of the lectin from the strains gs1 was studied with various erythrocytes, and the result showed that the lectin from the strains gs1 could agglutinate erythrocytes of rabbit, rat and mouse, but could not agglutinate erythrocytes of human (A\B\AB\O type) and chick. The strain gs1 should belong to Aspergillus according to its taxonomic characteristics of the colony on PDA medium.
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Received: 06 March 2008
Published: 02 July 2009
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