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| The Effects of Glycosyltransferase Genes Double Disruption in Ebosin Biosynthesis |
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Abstract Abstract The biosynthesis cluster (ste) of a novel exololysaccharide called Ebosin producing by Streptomyces had been identified previously. The results showed that the gene products of ste15 and ste22 were glucosyltransferase and rhamnosyltransferase respectively. In this study, the ste22 gene was disrupted with a double crossover via homologous recombination in the mutant strain Streptomyces sp. 139 (ste15 -). The mutant strain Streptomyces sp.139 (ste15 –ste22 -) was identified by Southern blot and gene complementation also performed. Compared with Ebosin, the glucose and rhamnose of EPS15-22m produced by Streptomyces sp.139 (ste15 –ste22 -) were reduced obviously, it’s Mp and the antagonist activity for IL-1R decreased. Glucose, rhamnose and the antagonist activity for IL-1R were recovered in EPS15-22c producing by the gene complemented strain. This study elucidated that genes ste15 and ste22 play essential roles in the formation of repeating units of sugars during Ebosin biosynthesis. The activities of Ebosin new derivatives produced by the mutants have been studied further both in vitro and in vivo.
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Received: 09 April 2009
Published: 02 July 2009
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