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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (06): 125-129    DOI:
    
Advance in application of PCR for sex identification and sex control
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Abstract  

As a rapid development biological technology, PCR has been widely used in many related fields such as sex identification for it is rapid, sensitive, convenient and specific. The PCR method used in sex identification varies from simple PCR, nested PCR to modified two-temperature gradient PCR. Besides the gene sequences such as Sry, which only locates in the Y Chromosome, gene sequences like zinc finger protein which present different gender characteristics between different genders were also detected. This has provided a brand-new primer designing thought and PCR method for sex identification. With the development of the study, the PCR technique is bound to become more mature on the application of sex identification and sex control. Much more progress will be made on promoting the application of PCR to other related fields at the same time.



Key wordsPCR technology;sex identification;sex control;progress     
Received: 22 December 2008      Published: 02 July 2009
Cite this article:

林博,朱化彬,郝海生,杜卫华,赵学明,王栋. Advance in application of PCR for sex identification and sex control. China Biotechnology, 2009, 29(06): 125-129.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I06/125

[1] Maxwell W M C, Evans G, Hollinshead F K, et al. Integration of sperm sexing technology into the ART toolbox. Animal Reproduction Science, 2004, 82(83): 79~95 [2] Graaf S P, Evans G, Maxwell W M C, et al. Birth of offspring of predetermined sex after artificial insemination of frozen–thawed, sexsorted and refrozen–thawed ram spermatozoa. Theriogenology, 2007, 67: 391~398 [3] 郭家明,朱化彬,王栋,等. Y精子分离纯度评价方法的研究进展.遗传 ,2008,30 (9): 1115~1120 Guo J M, Zhu H B, Wang D, et al. Progress on methods for purity assessment of separated chromosome X or Ybearing sperm. Hereditas, 2008,30 (9) :1115~1120 [4] Lu Y Q, Liang X W, Zhang M, et al. Birth of twins after in vitro fertilization with flowcytometric sorted buffalo(Bubalus bubalis) sperm. Animal Reproduction Science, 2006,3180~3185 [5] Chrenek P, Boulanger L, Heyman Y. Sexing and multiple genotype analysis from a single cell of bovine embryo. Theriogenology, 2001, 55: 1071~1081 [6] 王丽娟.PCR技术在牛早期胚胎性别鉴定中的应用.黑龙江动物繁殖,2007,15 (6): 17~20 Wang L J.Hei Longjiang Animal Reproduction, 2007, 15(6): 17~20 [7] 陈从英,黄路生,陈静波,等.牛早期胚胎性别鉴定体系的建立和优化.农业生物技术学报,2003,11(4):399~402 Chen C Y, Huang L S, Chen J B, et al.Journal of Agricultural Biotechnology, 2003, 11(4):399~402 [8] 张建新,岳文斌,赵宇军.山羊体外受精胚性别鉴定不同取样方法的研究.激光生物学报, 2004,13(2):120~123 Zhang J X, Yue W B, Zhao Y J.Acta Laser Biology Sinica, 2004,13(2):120~123 [9] Zhang T N,Lu J C,Lu C W, et al .A multiplex PCR assay for the detection of pathogenic genes of EPEC and EIEC. Journal of Northeast Agricultural University, 2006, 13(1):51~54 [10] 王昆,王栋,李魁英,等.利用多重PCR技术鉴别牛早期胚胎性别研究.中国奶牛,2008,4:25~26 Wang K, Wang D, Li K Y, et al.China Dairy, 2008, 4: 25~26 [11] Peura T, Hyttinen J M, Turunen M, et al. A reliable sex determination assay for bovine preimplantation embroyos using the polymerase chain reaction.Theriogenolohy, 1991, 44(2): 167~176 [12] 肖海霞,陈从英,陈静波,等.应用PCR技术鉴定牛早期胚胎性别方法优化的研究.云南畜牧兽医,2002,4:1~2 Xiao H X, Chen C Y, Chen J B, et al.Yunnan Journal of Animal Science and Veterinary Medicine, 2002, 4: 1~2 [13] 王晗,窦忠英.应用巢式及复合PCR对小鼠早期胚胎进行性别鉴定.西北农业学报,2005,14(4):158~161,165 Wang H, Dou Z Y.Acta Agriculturae BorealiOccidentalis Sinica, 2005,14(4):158~161,165 [14] Ren W, Yang F, Welch G, et al. An X–Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures. Reproduction, 2001, 121: 541~546 [15] 赵学明,朱士恩,侯云鹏,等. 双重套PCR对不同取样胚胎性别鉴定灵敏度测试.中国生物工程杂志,2005,25(3):17~22 Zhao X M, Zhu S E, Hou Y P, et al.China Biotechnology, 2005, 25(3):17~22 [16] Welch G R, Johnson L A. Sex preselection: Laboratory validation of the sperm sex ratio of flow sorted X and Ysperm by sort reanalysis for DNA. Theriogenology, 1999, 52: 1343~1352 [17] Brien O J K, Stojanov T, Crichton E G, et al. Flow cytometric sorting of fresh and frozenthawed spermatozoa in the western lowland gorilla. Am J Primatol, 2005, 66(4): 297~315 [18] Ramalho M D T, Aires B C A, Esper C R, et al. Sexing of murine embryos by developmental arrest induced by high titer HY antisera. Theriogenology, 2001,55:484 [19] Park J H, Lee J H, Choi K M. Rapid sexing of preimplantation bovine embryo using consecutive and multiplex polymerase chain reaction (PCR) with biopsied single blastomere.Theriogenology,2001,55:1843~1853 [20] Cha R S, Zarbl H, Keohavong P. Mismatch amplification mutation assay (MAMA): application to the cHras gene.PCR Methods and Applications, 1992, 2: 14~20 [21] 朱化彬,王栋,程金华,等.牛早期胚胎性别快速鉴别的研究.畜牧兽医学报,2005,36(12):1270~1274 Zhu H B, Wang D,Cheng J H, et al.Chinese Journal of Animal and Veterinary Sciences,2005,36(12):1270~1274 [22] 王栋,程金华,朱化彬,等.两温度梯度多重PCR鉴别牛早期胚胎性别的技术.遗传,2006,28(3):334~338 Wang D,Cheng J H,Zhu H B,et al.Hereditas, 2006,28(3):334~338 [23] Paula B, Munhoz S, Cristiane D, et al. Standardization of a duplex RTPCR for the detection of Influenza and Newcastle disease viruses in migratory birds.Journal of Virological Methods,2005,22: 125~130 [24] Nico B, Wim D W, Willy V, et al. Evaluation of nested PCR–DGGE (denaturing gradient gel electrophoresis) with groupspecific 16S rRNA primers for the analysis of bacterial communities from different wastewater treatment plants. Microbiology and Virology, 2006, 39(2): 101~112 [25] Gibson C W, Golub E, Hemld R, et al. Structure and expression of the bovine amelogenin gene. Biochemistry ,1991,30(4):1075~1079 [26] Gibson C W, Golub E E, Abranis W R, et al. Bovine amelogenin message heterogeneity: alternative splicing and Ychromosomal gene transcription.Biochemistry,1992,31(35):8384~8388 [27] Chen C M, Hu C L, Wang C H, et al. Molecular reproduction and development.1999,54(3):120~125 [28] 陈晓军,罗淑萍,吴阳升,等.应用AML基因进行牛性别鉴定的研究.中国奶牛,2005,3:7~9 Chen X J, Luo S P, Wu Y S, et al.China Dairy, 2005,3:7~9 [29] 幸宇云,陈从英,黄路生,等.应用牙釉蛋白(Aml)基因鉴别牛早期胚胎性别.农业生物技术学报,2005,13(6):759~762 Xing Y Y, Chen C Y, Huang L S, et al.Journal of Agricultural Biotechnology, 2005,13(6):759~762 [30] Van D V. Identity Testing: Applications of DNA Typing in Anatomic Pathology.Philadelphia, 2003.163~188
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