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| Screening and Preliminary Analysis on an Embryo Development-related Gene from Promoter Trapping Line in Rice |
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Abstract Seven promoter trapping lines, which GUS activity were detected in embryo, were found by screening 2227 lines from an indica promoter trapping pool. Of which one line accession NO.W9154 was further analyzed.A single copy T-DNA insertion was indicated in the W9154 line by Southern blotting and its T-DNA inserted locus was located in the third intron of an unknown protein gene in chromosome 3 in rice. Analysis on the upstream regulation sequence of the trapped gene revealed that it contained seed-specific promoter elements, such as RY motif、E-box、AACA and so on, besides general promoter elements TATA-box and CAAT-box. Further analysis was conducted on expression pattern of the candidate gene, and it was found that the candidate was only expressed in embryo and stem, but not other tissues in wild type rice. This result was completely consistent with that of the GUS expression in the trapping line w9154.Taken together,the analyses indicated that the trapped gene of the line w9154 might be an embryo development-related gene in rice.
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Received: 11 December 2008
Published: 02 July 2009
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Cite this article:
HU Chang-Quan-1, 3, LI Su-Yi-1, 2, LIU Hua-Qing-2, LIU Ci-Tao-2, ZHANG Hui-1, 2, BO Da-Ren-3, WANG Feng-1. Screening and Preliminary Analysis on an Embryo Development-related Gene from Promoter Trapping Line in Rice. China Biotechnology, 2009, 29(06): 108-112.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I06/108
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| ［1］ 蒋丽,齐兴云,龚化勤,等.被子植物胚胎发育的分子调控.植物学通报, 2007, 24 (3): 389398
Jiang L,Qi X Y,Gong H Q. Chinese Bulletin of Botany,2007, 24 (3): 389~398
［2］ Chen Xinbo, Mark Goodwin S, Liu Xionglun, et al. Mutation of the Resurrection1 locus of Arabidopsis reveals an association of cuticular wax with embryo development1. Plant Physiology, 2005, 139: 909~919
［3］ Nancy A Eckardt. Genetic and epigenetic regulation of embryogenesis. Plant Cell,2006,18:781~784
［4］ Pablo D Jenik, Rebecca E. Jurkuta J,et al. Interactions between the cell cycle and embryonic patterning in Arabidopsis uncovered by a mutation in DNA polymerase. Plant Cell,2005,17: 3362~3377
［5］ Junya Mizoi, Masanobu Nakamura, Ikuo Nishida . Defects in CTP:Phosphorylethanolamine cytidylyltransferase affect embryonic and postembryonic development in Arabidopsis. Plant Cell, 2006,18:3370~3385
［6］ YongHe Ding, NaiYou Liu,ZuoShun Tang. Arabidopsis glutaminerich protein23 is essential for early embryogenesis and encodes a novel nuclear PPR motif protein that interacts with RNA polymerase II subunit III. Plant Cell, 2006,18:815~830
［7］ Hall Q, Cannon M C. The cell wall hydroxyprolinerich glycoprotein RSH is essential for normal embryo development in Arabidopsis. Plant Cell ,2002,14: 1161~1172
［8］ Schrick K, Mayer U, Horrichs A,et al. FACKEL is a sterol C14 reductase required for organized cell division and expansion in Arabidopsis embryogenesis. Genes Dev,2000,14: 1471~1484
［9］ Xu J, Zhang H Y, Xie C H,et al.Embryonic factor 1 encodes an AM Pdeaminase and is essential for the zygote to embryo transition in Arabidopsis.Plant J,2005,42: 743~756
［10］ Han B W, Bingman C A, Mahnke D K, et al. Membrane association, mechanism of action, structure of Arabidopsis embryonic factor 1 (FAC 1). J Biol Chem, 2006,281: 14939~14947
［11］ Ding Y H, Liu N Y, Tang Z S,et al. Arabidopsis glutaminerich protein23 is essential for early embryogenesis and encodes a novel nuclear PPR motif protein that interacts with RNA polymerase II subunit III. Plant Cell,2006,18: 815~830
［12］ Xiao W, Custard K D, Brown R C, et al. DNA methylation is critical for Arabidopsis embryogenesis and seed viability. Plant Cell, 2006,18: 805~814
［13］ Jefferson R A. Assaying chimeric genes in plants:The GUS gene fusion system.Plant Molecular Biology Report, 1987,5:387~405
［14］ Gallagher S R. GUS Protocols:Using the GUS Gene as a Reporter of Gene Expression. San Diego: Academic Press, 1992. 213
［15］ 王关林,方宏筠.植物基因工程.第2版.北京:科学出版社,2002.744
Wang G L,Fang H J.Plant Gene Engineering.Beijing:Science Press,2002.744
［16］ Liu Y G, Robert F W. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from PI and YAC clones for chromosome walking. Genomics,1995,25:674~681
［17］ Liu Y G,Teruko O, Robert F W,et al. Efficient isolation and mapping of Arabidopsis thaliana TDNA insert junctions by thermal asymmetric interlaced PCR.The Plant Journal, 1995, 8 (3):457~463
［18］ 刘晓娜,付畅,黄永芬.种子特异性启动子研究进展,植物学通报,2007,24(2):218~225
Liu X N,Fu C,Huang Y F, Chinese Bulletin of Botany, 2007,24(2):218~225
［19］ 李吉涛,郭建春.种子特异性启动子的研究进展.安徽农业科学,2008,36(4):1382~1413
Li J T,Guo J C. Journal of Anhui Agricultural Sciences, 2008,36(4):1382~1413
［20］ Springer P S. Gene traps: tools for plant development and genomics.Plant Cell,2000,12:1007~1020
［21］ Krishnan A, Guiderdoni G, An G,et al. Mutant resources in rice for functional genomics of the grasses. Plant Physiology, 2009, 149:165~170
［22］ Springer P S,McCombie W R, Sundaresan V,et al.Gene trap tagging of prolifera,an essential MCM235like gene in Arabidopsis.Science,1995,268:877~880
［23］ Springer PS,Holding D R,Groover A,et al.The essential Mcm7 protein prolifera is localized to the nucleus of dividing cells during the G1 phase and is requied maternally for early Arabidopsis development.Development,2000,127:1815~1822
［24］ Dubreucq B,Berger N,Vincent E,et al.The Arabidopsis AtEPR 1 extensinlike gene is specifically expressed in endosperm during seed germination.Plant J,2000,23:643~652
［25］ Guiderdoni E, An G, Yu S M, et al.TDNA Insertion Mutants as a Resource for Rice Functional Genomics. In: Upadhyaya N M (ed) Rice Functional Genomics Challenges, Progress and Prospects. New York:Springer, 2007.181~221
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