[1] Jemal A, Siegel P, Ward E, et al. Cancer statistics.CA Cancer J Clin, 2007, 57:43~66 [2] Goldenberg M M. Trastuzumab, a recombinant DNAderived humanized monoclonal antibody, a novel agent for the treatment of metastatic breast cancer . Clin Ther, 1999, 21(2): 309 ~318 [3] Viani G A, Afonso S L, Stefano E J, et al. Adjuvant trastuzumab in the treatment of her2positive early breast cancer: a metaanalysis of published randomized trials .BMC Cancer, 2007, 7:153 [4] Yeon C H, Pegram M D. Anti-erbB-2 antibody trastuzumab in the treatment of HER2amplified breast cancer.Investigational New Drugs,2005,23:391~409 [5] Swinkels B W,van Ooyen A J,Bonekamp F J. The yeast Kluyveromyces lactis as an efficient host for heterologous gene expression .Antonie van Leeuwenhoek, 1993, 64(2): 187~201 [6] Dujon B, Sherman D, Fisher G, et al. Genome evolution in yeasts . Nature, 2004, 430:35~44 [7] 王凌雪.抗HER2人源化单克隆抗体在毕赤酵母中的高效表达及其产物分析.重庆:西南大学,食品科学学院,2008. Wang L X.Expression and Characterization of Humanized AntiHER2 Monoclonal Antibody in Pichia pastoris yeast.Chongqing:Southwest University,College of Food Science,2008. [8] 刘波.乳酸克鲁维酵母糖基工程研究.北京:军事医学科学院,生物工程研究所,2008. Liu Bo.The Research of Glycosylation Engineering in Kluyveromyces lactis.Beijing:Academy of Military Medical Science,Beijing Institute of Biotechnology,2008. [9] 刘阳,孙志伟,俞炜源等.HER2胞外域基因的克隆及其在大肠杆菌中的可溶表达.生物技术通讯,2005,16(3):252~254 Liu Y,Sun Z W,Yu W Y,et al.Letters in Biotechnology,2005,16(3):252~254 [10] Albert J J,van Ooyen, Prter Dekker, et al. Heterologous protein production in the yeast Kluyveromyces lactis.FEMS Yeast Res,2006 (6):381~392 [11] Swennen D, Paul M F, Vernis L, et al. Secretion of active antiRas singlechain Fv antibody by the yeasts Yarrowia lipolytica and Kluyveromyces lactis. Microbiology ,2002,148: 41~50 [12] Robin S, Petrov K, Dintinger T, et al.Comparison of three microbial hosts for the expression of an active catalytic scFv. Mol Immunol,2003,39(12):729~738 [13] Weiner L M, Adams G P. Monoclonal antibody therapy of cancer. Nature Biotechenology, 2005, 23(9):1147~1157 [14] 沈倍奋.治疗性抗体的改造.医学分子生物学杂志,2006,3(4):245~249 Shen B F. Journal of Medical Molecular, 2006,3(4):245~249 [15] Coloma M J, Clift A, Wims L, et al. The role of carbohydrate in the assembly and function of polymeric IgG. Mol Immunol, 2000, 37:1081~1090 [16] Mimura Y,Chcuch S,Chirlando R, et al. The influence of glycosylation on the thermal stability and effector function expression of human IgG1Fc: properties of a series of truncated glycoform. Mol Immunol,2000, 37:697~706 [17] Wright A, Morrison S L. Effect of glycosylation on antibody function: implication for genetic engineering. Tibtech,1997,15:26~32 [18] Choi B K, Bobrowicz P, Davidson R C, et al. Use of combinatorial genetic libraries to humanize Nlinked glycosylation in the yeast Pichia pastori.Applied Biological Sciences,2003,100(9):5022~5027 [19] Stephem R H, Bobrowicz P, Bobrowicz B, et al. Production of complex human glycoproteins in yeast. Science,2003,301:1244~1246 |