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Creation and Characteristics Research of HepG2/mdr1 Stable Cell Line |
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Abstract Eukaryotic expression plasmid pCI-neo-mdr1 which contains human multidrug resistance gene 1(mdr1), was constructed and transferred into human hepatocarcinoma HepG2 cells by use of liposome. G418 was used to screen the cells successfully with mdr1 and the selected cells was named HepG2/mdr1 Morphological and biological properties of HepG2/mdr1 cells were observed. The results show that the constructed HepG2/mdr1 cell line was high efficient and stationary in the expression of mdr1. The work was valuable and desirable for the establishment of multidrug resistant cell models, and for the study of MDR in human hepatoma. Furthermore, the work also provided a perfect model for the research of relationship between insulin resistance and MDR in hepatocarcinoma cells.To construct the eukaryotic expression plasmid pCI-neo-mdr1 containing multidrug resistance gene 1(mdr1), and transfer into human hepatocarcinoma cell line HepG2 cell by use the liposome, screening human hepatoma multidrug resistance (MDR) cell line HepG2/mdr1 with G418, and the biological property of HepG2/mdr1 cells was observed. The results showed that the HepG2/mdr1 cell line that we constructed is highly efficient and stationary in performance and the goal gene expression. This work was valuable and desirable for the study of MDR phenomenon and reversing measure of MDR, and exploring establish of MDR hepatocarcinoma cell line. Furthermore, The work also provided a perfect model cell for the research of relationship between insulin resistance and MDR in hepatocarcinoma cells.
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Received: 13 November 2008
Published: 06 May 2009
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