中国生物工程杂志

The culture conditions in 250 ml flask for the production of CoQ10 by Rhodopseudomonas palutris J001 were investigated. The results showed that the optimal initial pH, temperature, rpm of rotary shaker, medium volume in the flask and inoculum’s size were 6.5-7.5, 28-31℃, 100 rev min-1, 200 ml and 10%, respectively. The suitable carbon source was NaAc and suitable nitrogen sources were (NH4)2SO4 and NH4Cl. But the effects of phosphorus source on the cell growth and CoQ10 production were insignificant. With the aid of response surface method, the optimum concentrations of NaAc and (NH4)2SO4 were 5.39（g l-1）and 0.385（g l-1）for the growth of biomass, respectively, which the ratio of C/N was 14/1; and the optimum concentrations of NaAc and (NH4)2SO4 were 5.70（g l-1）and 0.365（g l-1）for the production of CoQ10, respectively, which the ratio of C/N was 15.6/1.