中国生物工程杂志

Previous results indicated that the abnormal development of haploid goldfish embryos is due to the expression obstruction of some regulation proteins.In search of the mechanism of abnormal development in goldfish gynogenetic haploid embryos, differential display proteomics techniques was employed to study protein changes among three different develomental stages time of haploid embryos called HE-1, HE-2 and HE-3. Proteins were sepatated by two dimensional electrophoresis, then image analysis was carried out using PDQuest image analysis software. 15 proteins were identified by MALDI-TOF/TOF. These results may be helpful in further studing developmental mechanisms of goldfish gynogenetic haploid embryos.