中国生物工程杂志

The single preimplantation embryo differential display reverse transcription polymerase chain reaction(SPEDDRT-PCR) was used to identify differential genes in 2-cell,4-cell,8-16-cell porcine parthenogenetic activated embryos.A total of 8 ESTs(expressed sequences tags) were found using SPEDDRT-PCR and reverse northern dot blot, one of which in 2-cell,four in 4-cell,and the other three in 8-16-cell embryo.All 8 ESTs were compared with nucleotide sequences deposited in the nr databasse and the dbEST database of Genbank using BLAST.DD3,DD4,DD5,DD6,DD7 and DD8 ESTs had their highly similar nucleotide sequences with ESTs existing in nucleotide databases but with unknown function.DD1 and DD2 ESTs had no significant similarity with exisiting genes or ESTs and were regarded as new ESTs. The two new ESTs were submitted to GenBank(accession numbers: EU545158, EU545159).This lays a foundation for further study on the mechanism of porcine preimplantation embtyo development.