construction of RNAi expression vectors by Fusion gene fragments of gbss,ssⅢ and ssⅡ from potato

China Biotechnology

2008, Vol. 28

Issue (8): 51-56 DOI:

construction of RNAi expression vectors by Fusion gene fragments of gbss,ssⅢ and ssⅡ from potato

Download:

HTML

PDF(1024KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract

Granule-bound starch synthase and soluble starch synthase are the key enzymes in starch synthesis. Their activity would decide starch quality via affecting the chain length, the ratio of amylose content to amylopectin and the structure of starch. We performed homology analysis on gbss, ssII and ssIII with software Blast and DNAStar followed by using 261bp fragments from gbss（1～261），244bp fragments from ssⅢ（2164～2407）and 281 bp fragments from ssⅡ（161～441）to make the fusion gene gbs3s2 with overlap PCR. Then we constructed the promoter CaMV 35S driven, containing 'forward gbs3s2 fusion fragments-reverse pdk intron-reverse gbs3s2 fusion fragments', plant siRNA expression vector, which lay the foundation for breeding a low gelatinization temperature potato.

Key words： RNA interference potato fused gene overlap PCR

Received: 10 March 2008 Published: 25 August 2008

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

. construction of RNAi expression vectors by Fusion gene fragments of gbss,ssⅢ and ssⅡ from potato. China Biotechnology, 2008, 28(8): 51-56.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I8/51

[1]	HE Li-heng,ZHANG Yi,ZHANG Jie,REN Yu-chao,XIE Hong-e,TANG Rui-min,JIA Xiao-yun,WU Zong-xin. Construction of Gene Co-expression Network and Identification of Hub Genes Related to Anthocyanin Biosynthesis Based on RNA-seq and WGCNA in Sweetpotato[J]. China Biotechnology, 2021, 41(9): 27-36.

[2]	FENG Zhao,LI Jiang-hao,WANG Jia-hua. Functional Analysis of RpRPL22, a Ribosomal Protein Homologous Gene, in the Symbiotic Nodulation Process of Robinia Pseudoacacia[J]. China Biotechnology, 2021, 41(7): 10-21.

[3]	Yu CHENG,Qiong SHI,Li-qin AN,Meng-tian FAN,Gai-gai HUANG,Ya-guang WENG. BMP7 Gene Silencing Inhibits Osteogenic Differentiation of Porcine Arotic Valve Interstitial Cells Induced by Osteogenic Induction Medium[J]. China Biotechnology, 2019, 39(5): 63-71.

[4]	Zhao FENG,Min-xia CHOU. *Functional Analysis of Rpfan37* in the Symbiotic Nodulation Process of Robinia Pseudoacacia**[J]. China Biotechnology, 2018, 38(5): 47-55.

[5]	DONG Wei-peng,ZHANG Shao-hua,XU Xiang,YAN Jiong. *The Effect and Mechanism of Down-Regulated Fsp27* Gene Combined with Myricetin on Lipolysis of 3T3-L1 Adipocytes**[J]. China Biotechnology, 2018, 38(12): 7-13.

[6]	LIU Yi-xuan, BIAN Zhen, MA Hong-mei. Progress and Prospect of Cancer Gene Therapy[J]. China Biotechnology, 2016, 36(5): 106-111.

[7]	HU Na, LIU Qing, TANG Zhao-yong, TANG He-jing, AO Lan, ZHAO Zi-hao, FANG Liao-qiong. *siRNA Inhibits the Growth and Migration of Mouse Melanoma by MMP-9* and FAK Gene**[J]. China Biotechnology, 2016, 36(5): 34-39.

[8]	ZHAO Zhi-wu, WANG Jun-shi, MA Min, ZHANG Shao-hua, YAN Jiong. *Effect of Down-regulated Perilipin 1* Gene Expression on Lipolysis of 3T3-L1 Adipocytes**[J]. China Biotechnology, 2016, 36(3): 17-22.

[9]	NIE Li-zhen, YU Xiao-xia, LI Guo-jing, SUN Jie, JIANG Chao, YU Zhuo. *Study on Transgenic Potato Contained AtCDPK1* Gene Drived by Rd29A Promoter**[J]. China Biotechnology, 2015, 35(11): 13-22.

[10]	XUE Yu-wen, LI Tie-jun, ZHOU Jia-ming, CHEN Li. The Application and Perspectives of Multi-target RNAi in the Research and Development of Gene Therapy[J]. China Biotechnology, 2015, 35(1): 75-81.

[11]	SU Lan, ZHANG Ping, WANGYANG Jun-qi, ZHONG Ru-gang. Progress on the Inhibition of Hepatitis B virus by siRNA Strategy[J]. China Biotechnology, 2014, 34(9): 102-107.

[12]	TANG He-jing, TANG Zhao-yong, LIU Long-xing, ZHANG Xiao-mei, WANG Yi-ting, FANG Liao-qiong. Effect of siRNA Combined-silencing MMP-9 and FAK on Invasion and Migration of Mouse Melanoma Highly Metastatic Cells B16F10 in vitro[J]. China Biotechnology, 2014, 34(9): 40-47.

[13]	HUANG Tian-qing, KONG Qing-ran, LI Yan, YU Miao, LIU Zhong-hua. The Effect of Insulin Receptor Substrates 1 and 2 Knockdown on Porcine Hepatic Glucolipid Metabolism[J]. China Biotechnology, 2014, 34(4): 27-35.

[14]	PANG Min, WANG Hai-long, GUO Min, GUO Rui. Construction of an Eukaryocyte Expression Vector of Human ANKRD49 and the Study of Function and RNA Interference Target of ANKRD49[J]. China Biotechnology, 2014, 34(10): 15-21.

[15]	ZHANG Hao-ran, ZENG Zhi-yong, CHEN Jun-min. The Influence of Downregulation of DEPTOR Expression by RNA Interference on the Proliferation and Apoptosis of Human Multiple Myeloma Cells in vitro[J]. China Biotechnology, 2013, 33(5): 13-21.

Viewed

Full text

Abstract

Cited

Shared

Discussed