Expression of functional mouseβ2-adrenergic receptor in the methylotrophic yeast Pichia pastoris and activity determination

China Biotechnology

2008, Vol. 28

Issue (8): 18-22 DOI:

Expression of functional mouseβ2-adrenergic receptor in the methylotrophic yeast Pichia pastoris and activity determination

Shuming Yang

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Abstract

Object: to obtain large quantities of β2-adrenergic receptor (β2AR) and detect β2-agonist.Method:Mouseβ2AR cDNA gene was amplified by PCR, then linked with EGFP and inserted into yeast expression vector pPICZαA. The reconstructed plasmid , PICZαDNAsGFP was transformed into Pichia pastoris KM71. After induction, a recombinant protein about 75kD in size was determined by western blot. 125ICYP was used to determine the activity of recombinantβ2AR. These results showed that the expressed protein is active.

Key words： β2-adrenergic receptor GFP Pichia expression system pPICZαA β2-agonist

Received: 28 February 2008 Published: 25 August 2008

Corresponding Authors: Shuming Yang

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Cite this article:

Shuming Yang. Expression of functional mouseβ2-adrenergic receptor in the methylotrophic yeast Pichia pastoris and activity determination. China Biotechnology, 2008, 28(8): 18-22.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I8/18

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