中国生物工程杂志

Objective：To observe the effect of retrovirally mediated RNA interference targeting CXCR4 of human cervix carcinoma Caski cells．Methods：Construct the fragment of CXCR4-siRNA, insert it into a retroviral vector which contain Green Flu Protein, named pSOS. Then, recombinated it by transfecting in PT67 cells, collect the supernatant of culture fluid, and use the supernatant transfect Caski cells. The expression of CXCR4 mRNA and protein were detected by Real-Time PCR and Western boltting. The abilities of invasion was detected by Matrigel invasion assay. Results: The retrovirus vectors pSOS-CXCR4 were constructed successfully. Compared with the negative control group, the inhibition rates of CXCR4 mRNA on the 24th, 48th and 72th hour were 29.9%, 56.8% and 62.8%, respectively. The inhibition rates of CXCR4 protein on the 24th, 48th and 72th hour were 43.6%,49.6% and 62.9%, respectively. And the the abilities of invasion was weaken when CXCR4's expression was inhibited. Conclusion: Retrovirally mediated RNA interference could effectively inhibit the expression of CXCR4 in human cervix carcinoma cells and weaken the the abilities of invasion.