中国生物工程杂志

Abstract Background The identification of mesenchymal stem cells (MSCs) have provided exciting prospects for cell-based regeneration after myocardic infraction.However cell therapy have inherent limitations such as low sur- vival rate of transplanted cells and insufficient cell number.It is known that cell-matrix adhesion plays a key role in cell proliferation,differentia-tion and survival,and chemokine CXCL12 may involvedin these prcesses.Thus we transfected mesenchymalstem cells with CXCL12 for local secretion of CXCL12 and then explored CXCL12 triggered adhesion of mesenchymal stem cells to extracellular matrix proteins.Methods Mesenchymal stem cells was transfected with CXCL12.αV andβ3 integrins content was evaluated by Western blot analysis. Cell adhesion to extracellular matrix was examined in-vitro and cell prolife-ration after transplantation in-vivo. Results Transfection of CXCL12 resulted in increased CXCL12 in situ.Increased CXCL12 induced elevated adhesion to fibronectin in-vitro and higher survival invivo.CXCL12 mediated adhesion and proliferation was established by αV andβ3 integrin subunits.Conclusions Che- moattractive mechanisms are involved in adhesion processes of mesenchymal stem cells.Increased CXCL12 leads to enhanced expression of αV andβ3 inte- grins,which may augment cell survival,proliferation and differrentiation.