中国生物工程杂志

DhaBCE from Klebsiella.pneumoniae was inserted into the yqhD upriver in the plasmid pET28(a+)-yqhD. The recombinant plasmid pET28(a+)dhaBCE-yqhD harboring the genes encoding dhaBCE and yqhD with a Shine-Dalgarno (SD) sequence was transformed into E. coli novablue to be co-expression. The results showed that after 16 hours calculated following the addition of inducer IPTG at induce temperature 28℃, the enzyme activity of glycerol dehydratase and yqhD. oxidoreductase in recombined strain could arrive 35 U/ mg and 82 U/ mg, individually, higher than the wild type. The yield of 1, 3-PD arrived at 39g/L when 55g/L of substrate glycerol was added in the fermentation culture. Excessive glycerol badly influence produce formation and inhibition of product on 1, 3-propenediol biotransformation existed.