中国生物工程杂志

Plants are constantly challenged by various biotic and abiotic stresses, such as disease, drought, high salinity, and low temperature in their natural environment, which cause adverse effects on the growth of plants and serious reductions in the quantity and quality of crops. AR2/ERF is a large family of transcription factors. Genes in the ERF family encode transcriptional regulators with a variety of functions involved in the developmental and physiological processes in plants. Three AP2/ERF family transcriptional regulators (BnaERF1, BnaERF2 and BnaERF3) were isolated from Brassica napus by in silico cloning method using the conserved domain amino acid sequence of Arabidopsis thaliana ERF as probe. Then, cDNA and deduced amino acid sequence, composition, hydrophobicty and hydrophilicity, physical and chemical characterization, phylogenetic tree, conserved domain sequences, function domain, molecular modeling, and folding state were predicted and analyzed. Then, we isolated BnaERF1, BnaERF2 and BnaERF3 genes from B. napus L. Huyou15 by RT-PCR and PCR using cDNA and DNA as template.