中国生物工程杂志

Object： To improve the genetic stability of HBV gene in transgenic mice．Methods：HBV transgenic mice were bred by backcross and double cross . The HBV gene expression and replication were studied with real-time PCR,ELISA and chemiluminescence．Results:The HBV transgenic mice have stably bred to 23rd generation．The serum HBsAg level is 4122.31±2044.74IU/ml; The rate of HBV transgenic mice whose serum HBV DNA reach 104-106copies/ml was 93.93%.The HBV replication and expression were improved markedly.There is no difference between male and female mice about serum HBsAg level. Conclusions:After breeding the HBV gene was expressed stably with high-level in transgenic mice.