中国生物工程杂志

Microbial lipases have been widely used in traditional industries, as well as in emerging biocatalysed areas owing to their ability to catalyze a variety of reactions in aqueous and non-aqueous media. Therefore, it is very important to enhance amount of lipase production by recombinant overexpression for meeting market demand. This article presents a critical review of main and novel strategies which have been employed for recombinant expression of microbial lipases, including codon optimization, fusion and co-expression, dual expression system based on hybrid promoters, homologous overexpression, cell surface displaying and high-throughput screening based on gene library of expression. These new technologies are gradually coming to the forefront in the recombinant expression of lipase, especially for cell surface displaying and high-throughput screening based on gene library of expression. Meanwhile, several recombinant expressions for representative microbial lipases were also introduced and discussed, which are available for consultation when attempting to overexpress any lipases by scientists and industrialists.