中国生物工程杂志

ABSTRACT：Objective：To search for the biomarkers for differential diagnosis between human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells. Methods：Laser capture microdissection was used to separate human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells, Proteome alterations were observed using two dimensional polyacrylamide gel electrophoresis(2-DE) technique and electrospray ionisation tandem mass spectrometry．Westernbloting was used to detect the different protein expressions in ESCC and esophageal epithelial cells, Results : The 2-DE patterns of human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells were established. 14-3-3 protein ε、S100A9 were identified differentially expressed between ESCC and esophageal epithelial cells. Conclusion ：Laser capture microdissection is a key technique in proteomic study which can effectively resolve the problem of irrelevant tissues．14-3-3 protein ε、S100A9 may serve as abiomarker for differential diagnosis of human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells.