Primary Study of Producing UMP by Overexpressing URA5 and URA3 Genes in Saccharomyces cerevisiae

China Biotechnology

2008, Vol. 28

Issue (12): 77-81 DOI:

Primary Study of Producing UMP by Overexpressing URA5 and URA3 Genes in Saccharomyces cerevisiae

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Abstract

To increase the biotransfomation efficiency from the orotic acid to the Uridine 5’-monophosphate(UMP), URA5 gene encoding Orotate phosphoribosytransferase was amplified from Saccharomyces cerevisiae BY4742 by PCR, then it was inserted into the expression vector pYX212(contained orotidine monophosphate decarboxylase gene URA3)and the pYX212-URA5 was transformed into Saccharomyces cerevisiae BJX12 by electroporation. The recombinant strain was elementarily used to convert orotic acid to UMP. The results showed that pYX212-URA5/BJX12 could accumulate 7mM UMP from 32mM orotic acid in 26h, significantly higher than both control groups pYX212/BJX12 ( 2.7mM ) and BJX12 ( 2.4 mM )

Key words： Biocatalysis Uridine 5’-monophosphate(UMP) Orotate phosphoribosytransferase(OPRTase) orotidine monophosphate decarboxylase(ODCase) Saccharomyces cerevisiae

Received: 25 July 2008 Published: 20 April 2009

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	LEI Gao-Xin
	CHEN Yong
	XU Lin
	YING Han-Jie

Cite this article:

. Primary Study of Producing UMP by Overexpressing URA5 and URA3 Genes in Saccharomyces cerevisiae. China Biotechnology, 2008, 28(12): 77-81.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I12/77

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