Optimization of fermentation medium with lactose as a inducer for high molecular weight recombinant spider silk protein

China Biotechnology

2008, Vol. 28

Issue (12): 41-46 DOI:

Optimization of fermentation medium with lactose as a inducer for high molecular weight recombinant spider silk protein

Download:

HTML

PDF(996KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract

The fermentation culture media for pNSR32/BL21(DE3) was investigated with shake culture method.Based on M9 medium and for the induction of lactose ,so as to determine the optimal concentration of ingredients of medium. The optimized culture medium was obtained by orthogonal and individual factor design. Optimized prescription of culture medium was investigted for the fermentation to produce the high molecular weight recombinant spider silk proteins. The optimum culture medium consisting of 0.3% glycerol,3% yeast,0.75% tryptone, 0.05%(NH4)2SO4 and little inorganic salt that was found to benefit for the growth and expression of recombinant spider silk proteins ,and to make the production level attain at 24.0%.

Key words： BL21(DE3) recombinant spider silk protein lactose Medium optimization

Received: 27 August 2008 Published: 20 April 2009

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	ZHENG Jian
	WU Lin-Lin
	LI Min

Cite this article:

. Optimization of fermentation medium with lactose as a inducer for high molecular weight recombinant spider silk protein. China Biotechnology, 2008, 28(12): 41-46.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I12/41

[1]	WANG Xiao-jie,MENG Fan-qiang,ZHOU Li-bang,LV Feng-xia,BIE Xiao-mei,ZHAO Hai-zhen,LU Zhao-xin. Breeding of Brevibacillin Producing Strain by Genome Shuffling and Optimization of Culture Conditions[J]. China Biotechnology, 2021, 41(8): 42-51.

[2]	Hai-jiao JI,Wen-lei LI,Rui-jing Huang,Jian LI,Han-mei XU. Anti-CD20rh MAb Quality Evaluation and Monoclonal Cell Line Screening[J]. China Biotechnology, 2018, 38(8): 34-40.

[3]	Yi-ying WANG,Hai-rong CHENG. *Cell Surface-Displaying the Lactose Hydrolase on Yarrowia lipolytica: a New Approach to Lactose Hydrolysis*[J]. China Biotechnology, 2018, 38(8): 41-49.

[4]	YIN Shu-xian, ZHAO Yue-hua, LIU Chao, LV Zhan-jun, WANG Xiu-fang. Construction and Expression of Engineering Bacteria Producing Humanized Alu RNA[J]. China Biotechnology, 2017, 37(7): 88-96.

[5]	LI Guo-kun, GAO Xiang-dong, XU Chen. Advances on Mammalian Cell Expression System[J]. China Biotechnology, 2014, 34(1): 95-100.

[6]	LIU Bo-ning. The Lasted Development of Large Scale Cell Culture Technology for Commercial Antibody Manufacture[J]. China Biotechnology, 2013, 33(7): 103-111.

[7]	ZHOU Guang-qi, MA Peng-bo, LIU Qiao, QUAN Chun-shan, FAN Sheng-di. *Optimization of Culture Medium and Prediction of Antibacterial Activity by Bacillus Amyloliquefaciens* Q-426 Fermentation**[J]. China Biotechnology, 2013, 33(11): 21-26.

[8]	CHEN Jie, WEI Hong-gang, LUO Yuan-chan, ZHANG Dao-jing, LI Shu-lan, TIAN Li, LI Yuan-guang. *Medium Optimization for the Production of New Antifungl Cyclic Lipopeptide Marinhysin A by Bacillus Marinus* B-9987**[J]. China Biotechnology, 2013, 33(1): 84-89.

[9]	WANG Chen, LI Sha, XU Hong, WEI Yan, CAI Heng. *Optimization of Fermentation Medium of Sucrose Isomerase by Recombinant Escherichia coli* through Response Surface Method**[J]. China Biotechnology, 2011, 31(04): 92-97.

[10]	RUAN Wen-Bing, CHEN Bi-Qin, CHEN Su-Hua, CHEN Bing-Mei, HU Xiao-Beng. Optimization of (R)-Mandelic acid Fermentation Medium by Using Response Surface Methodology[J]. China Biotechnology, 2010, 30(08): 112-117.

[11]	DENG Yong-Kang-1, TUN Min-Lu-2, LIU Cheng-Bang-1, DU Lin-Fang-1, WU Li-Li-1, LI Man-1, MENG Yan-Fa-1. Expression of recombinant uricase in E.coli JM109(DE3)Induced by lactose[J]. China Biotechnology, 2009, 29(07): 74-79.

[12]	. Expression of Plant Monellin in Escherichia coli Induced by Lactose[J]. China Biotechnology, 2008, 28(3): 53-58.

Viewed

Full text

Abstract

Cited

Shared

Discussed