中国生物工程杂志

Abstract The Saccharomyces cerevisiae cell-surface display expression system is one kind of eucaryotic display system which expresses heterogeneous protein on the cell surface by immobilization. The target protein is immobilized with GPI anchor and expressed on the cell surface after fusion gene that combines gene encoding protein and the specific carrier gene is introduced into yeast cells. It can be utilized in the fields of biocatalyst, cell-absorbent, live vaccine, environment management, protein library screening, high affinity antibody, biosensor, the antigen/antibody library construction, cancer diagnosis and so on. This mini-review describes molecular display using Saccharomyces cerevisiae and its basic principles, present research situation, various applications and development prospects.