System of intein-mediated PHB purified human antimicrobial peptide LL-37

China Biotechnology

2008, Vol. 28

Issue (11): 72-76 DOI:

System of intein-mediated PHB purified human antimicrobial peptide LL-37

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Abstract

The new intein-mediated PHB purify protein system is a high expression, automatic cutting, for purification, low-cost protein purification system,it is conducive to large-scale protein purification. This research choose human antibacterial peptide LL-37 as the purification objects,which is poison to prokaryotic cell.We construct intein-mediated PHB purified human antimicrobial peptide LL-37 system through genetic engineering technology and use this system to purify LL-37. The results show that this system can highly express LL-37 fusion protein and purifiy the product as same size with expectations

Key words： Protein expression Poly-β-hydroxybutyrate (PHB) Containing peptides Human antibacterial peptide LL-37

Received: 26 June 2008 Published: 20 April 2009

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	GU Yin-Xia
	ZHOU Hua-Zhang
	SONG Zhen-Wei
	YU Yu-Jiong

Cite this article:

. System of intein-mediated PHB purified human antimicrobial peptide LL-37. China Biotechnology, 2008, 28(11): 72-76.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I11/72

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