中国生物工程杂志

Soft rot caused by Erwinia carotovara.subsp.carotovora (Ecc) is a very serious disease in the world. Owing to the absence of natural resistant resources, the work moves slowly. Arabidopsis thaliana became the main target material for studying the mechanism of resistance to Ecc. In this paper, we selected 29 Arabidopsis accessions, then established the disease classification standards, and took the disease index at 48 hours post inoculated as the evaluation criteria of resistance to Ecc. The results showed that the ecotype CS906 was resistant and the ecotype CS20 was highly susceptive. By comparing gene expression changes of AOS, ERF1-1, PR1, PDF1.2 and PAL1 between the resistant and susceptible accessions at different time points after inoculation, we found that SA、ET and MJ signal transduction pathways were involved in the defense response of Arabidopsis to Ecc, and gene expression model was similar in CS906 and CS20, but the expression levels of genes in CS906 were higher than that in CS20. Upper results will facilitate the study on the resistance mechanism of Arabidopsis to Ecc.